Kyowa Hakko Kogyo Co., Ltd.

Agency of Industrial Science and Technology

Kyowa Hakko Kogyo

Kyowa Hokkoh Kogyo Co., Ltd.

Michio Shiomi

Ryoichi Katsumata

Ryuichiro Kurane

Haruhiko Yokoi

Hideki Murata

Shuichi Ishino

Yasuhiro Nohata

Takashi Ohshiro

Seiji Sugimoto

Akira Yotsuji

Tadao Tateba

Satoshi Iwata

Polysaccharide and a method of producing it

The present invention provides a process for producing trans-L-hydroxyproline, which comprises culturing a microorganism which is capable of decomposing amino acids other than trans-L-hydroxyproline but which is substantially incapable of decomposing trans-L-hydroxyproline in a culture medium containing collagen hydrolyzate, and recovering trans-L-hydroxyproline from the resulting culture. The process enhances the content of trans-L-hydroxyproline based on the total weight of amino acids contained in collagen hydrolyzate, and enables efficient production of trans-L-hydroxyproline which is useful as a starting material for the synthesis of medicines.

Process for producing trans-L-hydroxyproline

Disclosed is a process for producing crystals of a salt of an acidic amino acid and a basic amino acid, which comprises adding a water-miscible solvent to an aqueous solution of the salt to prepare a solution 90% or more saturated or supersaturated with the salt, heating the resultant solution under reflux until the formation of crystals stops, adding gradually a water-miscible solvent to the solution under reflux to form crystals of the salt, and recovering the crystals from the resultant mixture.

Process for producing crystals of salt of acidic amino acid and basic

A method for purification of mitomycin C comprises;

Method for purification of mitomycin C

Growing community of inventors

Hofu, Japan

Michio Shiomi