Takara Shuzo Co., Inc.

Takara Bio Inc.

Michio Hagiya

Ikunoshin Kato

Nobuto Koyama

Eiji Nishiyama

Takanari Tominaga

Hiroaki Sagawa

Tatsuji Enoki

Hua-Kang Wu

Kiyozo Asada

Takashi Uemori

Hiroyuki Mukai

Yoshimi Sato

Hiromu Ohnogi

Kazue Miyake

Osamu Takeda

Eiji Kobayashi

Katsushige Ikai

Jun Tomono

Hideto Chono

Junko Yamamoto

Tomoko Fujita

Mariko Moriyama

Haruhisa Sawaragi

Mariko Okawa

A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.

Method for amplifying nucleic acid sequence

A DNA synthesis method with a shortened time period required for DNA synthesis by polymerase chain reaction (PCR), characterized in that a DNA polymerase is used in an amount effective for providing more than 10 ng of amplified DNA fragments of about 2 kb per 50 &mgr;l of a reaction mixture, when PCR is carried out under the following conditions (A) and (B): (A) reaction mixture: 50 &mgr;l volume of a reaction mixture comprising DNA polymerase, 1 ng of genomic DNA from and 10 pmol each of primers Eco-1 and Eco-2 (nucleotide sequences of the primers Eco-1 and Eco-2 being shown in SEQ ID NOs: 10 and 11 of Sequence Listing, respectively); and having a composition suitable for the DNA polymerase; and (B) reaction conditions: 35 cycles of PCR, wherein one cycle consists of 99&deg; C., 1 second-66&deg; C., 7 seconds; a kit for DNA synthesis usable for the DNA synthesis method; and an article of manufacture of a PCR agent. According to the present invention, the procedures in the genetic engineering studies and industries involved with PCR can be speeded up.

Method for synthesizing DNA

An antirheumatic agent which is characterized in containing at least one compound selected from a group consisting of 4,5-dihydroxy-2-cyclopenten-l-one represented by the following formula &lsqb;I&rsqb; and an optically active substance and a salt thereof as an effective component.

Antirheumatic agents

An antiallergic agent which is characterized in containing at least one compound selected from a group consisting of 4,5-dihydroxy-2-cyclopenten-1-one represented by the following formula &lsqb;I&rsqb; and an optically active substance and a salt thereof as an effective component.

Antiallergic agents

A therapeutic or preventive agent for diabetes mellitus which is characterized in containing at least one compound selected from a group consisting of 4,5-dihydroxy-2-cyclopenten-1-one represented by the following formula &lsqb;I&rsqb; and an optically active substance and a salt thereof as an effective component.

Diabetes remedies

A compound represented by the following formula &lsqb;I&rsqb; or an optically active substance or a salt thereof.

Compounds

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Otsu, Japan

Michio Hagiya