Mitsui Toatsu Chemicals, Incorporated

Koichi Kawamura

Masaru Honjo

Akira Nakayama

Michiko Hori

Kazunori Ando

Yoshio Furutani

Keiko Fukazawa

Modified HV1-type hirudinin which valine at the N-terminal end of the HV1-type hirudin and aspartic acid at the 5th residue of the N-terminal end were replaced with alanine and glutamic acid, respectively. A secretion plasmid into which a DNA sequence coding for a precursor with an addition of a secretion signal of neutral protease of Bacillus amyloliquefaciens at the N-terminal end of this modified HV1-type hirudin is integrated is introduced into bacteria of the genus Bacillus and the precursor is expressed intracellularly. The modified HV1-type hirudin can be efficiently secreted extracellularly while maintaining its high thrombin inhibiting activity.

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Mobara, Japan

Michiko Hori