Kaneka Corporation

Dsm IP Assets B.v.

Daiichi Fine Chemical Co., Ltd.

Michihiko Kataoka

Sakayu Shimizu

Yoshihiko Yasohara

Noriyuki Kizaki

Junzo Hasegawa

Masaru Wada

Keiji Sakamoto

Tadanori Morikawa

Souichi Morikawa

Shinji Kita

Teruaki Takesue

Takahisa Nakai

Kazuya Tsuzaki

Miho Yano

Kazuhiko Yamamoto

Keiko Kita

Hiroshi Kawabata

Masahiro Funaki

Masashiro Funaki

The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus, a DNA encoding the polypeptide and a transformant that produces the polypeptide. The present invention moreover relates to a method of producing (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol utilizing the polypeptide or the transformant. Using the polypeptide or transformant of the present invention, optically active alcohols such as (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and the like can be produced efficiently.

Carbonyl reductase, gene thereof and method of using the same

Disclosed is an isolated DNA comprising a nucleotide sequence coding for an enzyme having enone reductase activity wherein the enzyme is characterized by the following physico-chemical properties: (a) molecular mass: 61,300&#xb1;5,000 Da (estimated using gel filtration, consisting of one subunit); (b) co-factor: NADPH and NADH; (c) substrate specificity: active on &#x3b1;,&#x3b2;-unsaturated ketons; (d) optimum temperature: 55-60&#xb0; C. at pH 7.4; and (e) optimum pH: pH 4.5-8.5.

Enone reductase gene and microbial production of levodione

An isolated enone reductase characterized by a molecular mass of 61300+/&#x2212;5000 Da; NADPH and NADPH as co-factor; a temperature optimum of 55&#x2013;60&#xb0; C. at pH 7.4; a pH optimum of 4.5&#x2013;8.5 and substrate specificity on &#x221d;,&#x3b2;-unsatured ketones; a process for producing it from a microorganism and a process for producing levodione from ketoisophorone using such reductase.

Enone reductase and methods of making and using thereof

A protein, which is an aminoketone asymmetric reductase, having an effect of producing d-pseudoephedrine by acting on 1-2-methylaminopropiophenone, and having the following physiochemical properties:

Aminoketone asymmetric reductase and nucleic acid thereof

Process for the production of optically active &bgr;-amino alcohols

An enzyme having carbonyl reduction activity of reducing a carbonyl compound asymmetrically to produce an optically active alcohol, a DNA coding the enzyme, a plasmid having the DNA, a transformant which is a cell transformed with the plasmid, and a production method of an optically active alcohol using the enzyme and/or the transformed cell are provided.

Carbonyl reductase enzyme and methods for its use

Gene encoding carbonyl reductase, and methods for its use

Growing community of inventors

Kyoto, Japan

Michihiko Kataoka