Toyo Kohan Co., Ltd.

Other

Nihon Parrkerizing Hiroshima Co., Ltd

Nihon Parkerizing Hiroshima Co., Ltd.

Michifumi Tanga

Kojiro Takahashi

Hirofumi Yamano

Hiroshi Okamura

Mitsuyoshi Ohba

Osamu Takai

Koichi Hirayama

Kenichi Takagi

Shuuichi Kamei

Kaoru Yamakawa

Takahiro Kitagawa

Teruhisa Icihara

Shyuichi Kamei

The present invention relates to a unit to be used for detection of interactions of biologically relevant molecules using a carrier on which the biologically relevant molecules are immobilized, comprising:

Method and unit for detection of interactions of biologically relevant molecules

It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.

Solid support having electrostatic layer and use thereof

A method for detecting hybridization between a probe polynucleotide and a target polynucleotide on a microarray is presented. The method may be used to determine the accuracy of hybridization and possible causes of inaccuracy, such as insufficient washing or deterioration of the microarray.

Method for detecting a position of probe on microarray

It is intended to provide a method of reusing a DNA-immobilization substrate whereby the expensive DNA-immobilization substrate can be efficiently utilized and a reusable DNA-immobilization substrate having the same performance as a new product without any trouble in practical use can be provided. Namely, a method of reusing a DNA-immobilization substrate characterized in that, to remove DNA from a DNA-immobilization substrate carrying the DNA immobilized by an acid-amide bond via an oligonucleotide to thereby enable the immobilization of a fresh DNA, the acid-amide bond between the substrate and the DNA is hydrolyzed with an acid or an alkali.

Method of reusing DNA-immobilization substrate

It is intended to provide a method whereby DNA can be conveniently and rigidly immobilized on a substrate at such a high density as achieved by the conventional methods and a method of accurately detecting DNA. A substrate activation kit comprising a phosphate buffer (pH6), a solution A (an aqueous solution) of N-hydroxysuccinimide and a solution B (a dioxide solution) of 1-[3-(dimethylamino) propyl]3-ethylcarbodiimide; and a method of detecting DNA characterized by comprising spotting DNA on a substrate having been activated by using the above activation kit and then hybridizing the spotted DNA with fluorescence labeled DNA.

Substrate activation kit and method for detecting DNA and the like using the same

Method and apparatus for constructing a cDNA library by hybridizing mRNA with oligo (dT) on a support and treating with a reverse transcriptase to immobilized complementary DNA, or for constructing a gDNA library by ligating a double-stranded chromosomal DNA library with an oligonucleotide on a support having a restriction enzyme site and then immobilizing the gDNA library.

Methods for constructing DNA library and support carrying DNA library immobilized thereon

Substrates for producing a DNA library are produced by immobilizing DNA onto a suitable substrate having excellent thermal conductivity. A surface of the substrate is chemically modified with a radical having a terminal polar radical. DNA can be amplified by the PCR method in a short period of time using substrates of the present invention onto which DNA is immobilized.

Substrates for immobilizing and amplifying DNA and DNA-immobilized chips

Supports for immobilizing DNA or the like

A microchip for chemical reaction capable of rapidly performing an experiment irrespective of the types of the chemical substrates used for the experiment under various experimental conditions, wherein a horizontal communication path comprising a plurality of chemical reaction pool portions and grooves communicating these chemical reaction pool portions to each other is formed in the surface of a diamond substrate of minute size forming a substrate, a vertical communication path comprising through-holes vertically communicating the chemical reaction pool portions to each other is provided in the diamond substrate , and an opening/closing valve is installed at a communicating portion, and further heating/cooling portions such as a Peltier device are installed at those positions corresponding to the chemical reaction pool portions , respectively, whereby a variety of experiments can be performed in a very small space without being restricted by the types of chemical substrates and by varying the experimental conditions.

Micro-chip for chemical reaction

A purpose of the present invention is to provide a gene amplification apparatus with simple operation, wherein the apparatus can heat and cool gene rapidly and a thermal cycle can be operated in a relatively short period and a method thereof, a method for preparing immobilized DNA library suitable to the above apparatus and a method for comparing genes systematically. The apparatus for preparing immobilized DNA library according to the present invention comprises a reaction body on which a grooved portion for receiving a container is formed, a cap portion provided at an upper portion of the reaction body and a container formed by substrates for immobilizing DNA, wherein the cap portion including means for heating/cooling and means for cooling

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Yamaguchi-ken, Japan

Michifumi Tanga