Novo Nordisk A/s

Michi Egel-Mitani

Per Balschmidt

Jan Markussen

Ivan Verner Diers

Allan Svendsen

Kim Borch

Ib Groth Clausen

Ib Jonassen

Mogens Trier Hansen

Thomas Borglum Kjeldsen

Shamkant Anant Patkar

Thomas Hoeg-Jensen

Jakob Brandt

Knud Vad

Svend Kaasgaard

Klaus Nyegaard Kristiansen

Jure Piskur

Aradhana Srivastava

Shamkant Anant Pathar

Henrik Mo Slashedllgaard

The present invention related to a method of producing polypeptides in transformed host cells by expressing a precursor molecule of the desired polypeptide which are to be acylated in a subsequent in vitro step. The invention is also related to DNA-sequences, vectors and transformed host cells for use in the claimed method. Further, the present invention is related to certain precursors of the desired polypeptides and certain acylation methods. The invention provides a method for making polypeptides being preferentially acylated in certain lysine &#x3b5;-amino groups.

Method for making acylated polypeptides

The invention describes industrial fermentation of a Saccharomyces yeast species for production of a heterologous product encoded by a plasmid or DNA contained in said Saccharomyces yeast species which method utilizes the substrate more efficiently and without fermentative metabolism resulting in formation of ethanol and other unwanted primary products of fermentative activity whereby high yields of the heterologous product are obtained. The Saccharomyces yeast species is preferably a Crabtree negative Saccharomyces species in particular

Method for the production of heterologous polypeptides in transformed yeast cells

The present invention relates to a novel method for the production of short chain polypeptides, including polypeptides having up to 3 disulfide bonds and/or structures rich in basic amino acid residues, and open structured short chain polypeptides, e.g. glucagon, glucagon like peptides and their functional analogues, in genetically modified yeast cells, said genetically modified yeast cells, and a method for the preparation of said yeast cells.

Method for the production of polypeptides

C. antarctica lipase variants

A DNA sequence encoding a lipase having the amino acid sequence shown in SEQ ID NO:2. The lipase has a trypsin-like catalytic triad including an active serine located in a predominantly hydrophobic, elongated binding pocket of the lipase molecule and, located in a critical position of a lipid contact zone of the lipase structure, an amino acid residue different from an aromatic amino acid residue, which amino acid residue interacts with a lipid substrate at or during hydrolysis, in which lipase variant of the amino acid residue has been replaced by an aromatic amino acid residue so as to confer to the variant an increased specific activity as compared to that of the parent lipase.

C. antarctica lipase and lipase variants

Process for the production of secondary metabolites

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Vedbaek, Denmark

Michi Egel-Mitani