Quest Diagnostics Investments, Inc.

3m Innovative Properties Company

Diasorin S.p.a.

Focus Diagnostics, Inc.

Michelle M Tabb

Maurice Exner

Jules Chen

Michael Aye

Peter Lee

Huong Mai

Lakshmi Nair

Lilly I Kong

Ming-Chou Lee

Fan Chen

Luca Jacky

Yin-Peng Chen

Albert Castro

Maria Vestal

Heather Vincent

Kristin Ramos

Raymond Huang

Peter D Ludowise

Ning Lu

Kyle Armantrout

David A Whitman

Lucien A E Jacky

Jose Matud

Larry McCarthy

Emberlee Eleazar

Lucien Jacky

Lucien AE Jacky

Kyle C Armantrout

The present disclosure provides methods for determining whether a patient exhibiting pertussis-like symptoms will benefit from treatment, with therapeutic agents that inhibit. These methods are based on detecting, andin a biological sample by assaying for the presence of the IS481, IS 1001, and hIS1001 target repeat elements, respectively. Kits for use in practicing the methods are also provided.

Methods for detecting

The present disclosure provides methods and compositions for determining whether a patient exhibiting acute gastroenteritis will benefit from treatment with therapeutic agents that inhibit Norovirus genogroup I (GI) or Norovirus genogroup II (GII). The methods disclosed herein are based on detecting Norovirus genogroup I (GI) and Norovirus genogroup II (GII) in a stool sample without extracting viral nucleic acids from a clinical specimen prior to performing real-time reverse transcription PCR. Kits for use in practicing the methods are also provided.

Methods for detecting norovirus

Provided herein are methods for identifying the presence or absence of a target nucleic acid from a microorganism using direct amplification without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids.

Direct amplification and detection of viral and bacterial pathogens

Disclosed are methods of identifying a methicillin-resistant(MRSA) in a sample wherein the methods involve detecting a-specific nucleic acid sequence, mecA and mecC, in the sample. Kits for determining the presence of MRSA in a sample are also provided.

Detection of methicillin-resistantin biological samples

Methods for detecting Norovirus

Disclosed herein are methods and compositions for detectingandby detecting the presence of the IS481 and IS1001 genomic insertion sequences, respectively.

Bordetella detection assay

Provided herein are methods for determining the presence or absence of an enteroviruses and parechoviruses in a biological sample. The methods involve identifying the presence or absence of a target nucleic acids from the viruses using direct amplification from a biological sample without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids. Also provided are methods of diagnosis using the methods provided and compositions and kits for the practice of the methods.

Molecular detection of enterovirus and parechovirus

Disclosed are methods for identifying the presence or absence of a target nucleic acid from one or more organisms in a biological sample, said method comprising: (a) spinning the sample at a rotational velocity sufficient to pellet cellular debris and fluorescence inhibitors present within the sample and reduce fluorescence interference or quenching in the sample; and (b) directly amplifying and detecting the target nucleic acid in the sample.

Amplification and detection of nucleic acids in a biological sample

detection assay

The present invention generally relates to a molecular test of Influenza A, Influenza B, Respiratory Syncytial Virus A, and Respiratory Syncytial Virus B in order to identify patients with a viral infection. Accordingly methods and compositions are disclosed to determine the presence or absence of a viral pathogen in a sample containing one or more target nucleic acids from the M gene of Influenza A, Influenza B, Respiratory Syncytial Virus A, and/or Respiratory Syncytial Virus B.

Multiplex detection assay for influenza and RSV viruses

Growing community of inventors

Santa Clara, CA, United States of America

Michelle M Tabb