The Australian National University

Biotechnology Australia Pty, Ltd

Michell A Clark

Philip Ralph Lehrbach

Toni Marie Antalis

Neil H Goss

Peter L Devine

Thomas M Barnes

Jeffrey P Golder

Ross W Stephens

Minactivin (also known as Plasminogen Activator Inhibitor-2 [PAI-2]), a protein inactivator of urokinase-type plasminogen activator, has been shown to be a natural inactivator of this plasminogen activator which is associated with invasive tumors, and is therefore indicated as a crucial element in the body's normal defense against tumor invasion and metastasis. It may be produced by the cultivation of minactivin-producing cells in vitro, and recovery of the cell culture supernatant. By controlling the culture conditions, the protein minactivin may be produced in a partially purified form which may be used for diagnosis and treatment of tumors. The specification discloses purification of biologically active native minactivin, as well as peptides derived from minactivin and their amino acid sequences. The specification also discloses methods for production of PAI-2 by recombinant DNA technology, characterization of a PAI-2 gene sequence, and expression and purification of large quantities of biologically active PAI-2 from a recombinant host.

Human PAI-2

Growing community of inventors

Crows Nest, Australia

Michell A Clark