Nestec S.a.

Michele Delley

Beat Mollet

Herbert Hottinger

Fabrizio Arigoni

Raymond David Pridmore

Jacques Edouard Germond

Francis Foata

Luciane LaPierre

Ivana Jankovic

Raymond-David Pridmore

The present invention generally relates to the field of probiotic bacteria. In particular, the present invention relates to natural derivatives of thestrain CNCM I-1225 with improved properties. For example, the present invention relates to a natural derivative of thestrain CNCM I-1225 that is deficient in D-lactic acid production and exhibits an improved viability during product storage.

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Acomprising a genome that is customized so as to lack an operable functional gene is disclosed. A method of making such cells is also disclosed. The method is used to makecells that lack certain functional antibiotic resistance genes, such as tetW, and are sensitive to antibiotics such as tetracycline.

A recombinant strain of L. johnsonii which has capacities of surviving passage through the intestine, adhering to human intestinal cells and increasing phagocytosis of macrophages and which has a gene sequence code for D-lactate dehydrogenase which is a modified L. johnsonii gene sequence code and which produces only L(+)-lactate.

Recombinant sequence-modified L. johnsonii bacteria which produce only   +

Strains of the Lactobacillus delbrueckii species are identified and distinguished from other species by a DNA probe containing an EcoRI fragment, or subfragment thereof, of the plasmid pY85, which hybridizes specifically to chromosomal DNA of strains of the L. delbrueckii species.

DNA probe for lactobacillus delbrueckii

Strains of the Lactobacillus delbrueckii species are identified by a probe having a DNA fragment which hybridizes specifically to chromosomal DNA of strains of the L. delbrueckii species.

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Savigny, Switzerland

Michele Delley