Fidia Farmaceutici S.p.a.

Fidia Farmaceuticals S.p.a.

Michele Caputo

Giovanni Gennari

Susanna Vaccaro

Christian Cuppari

The present invention claims a novel process for the production and purification of microbial collagenase (Microbial Collagenase EC 3.4.24.3) produced by the non-pathogenic aerobic bacteriumchemovar. iophagus (NCIMB Number: 11038, synonym LMG 3418, hereinafter called), which said process provides high production levels of collagenase with a stable, reproducible, cheap fermentation process. The collagenase produced fromaccording to the process described herein also presents a specific activity superior to that of other microbial collagenases, is stable in aqueous solution, and can be frozen without significant damage. A further subject of the present invention is pharmaceutical compositions containing collagenase obtained according to the production and purification process described, for the purpose of therapeutic treatment of disorders characterised by collagen accumulation or for the treatment of blemishes/imperfections that benefit from reducing local collagen accumulations.

Process for the production and purification of the collagenase enzyme from

The present invention claims a novel process for the production and purification of microbial collagenase (Microbial Collagenase EC 3.4.24.3) produced by the non-pathogenic aerobic bacteriumchemovar.(NCIMB Number: 11038, synonym LMG 3418, hereinafter called), which said process provides high production levels of collagenase with a stable, reproducible, cheap fermentation process. The collagenase produced fromaccording to the process described herein also presents a specific activity superior to that of other microbial collagenases, is stable in aqueous solution, and can be frozen without significant damage. A further subject of the present invention is pharmaceutical compositions containing collagenase obtained according to the production and purification process described, for the purpose of therapeutic treatment of disorders characterised by collagen accumulation or for the treatment of blemishes/imperfections that benefit from reducing local collagen accumulations.

Process for the production and purification of the collagenase enzyme from vibrio alginolyticus

The present invention claims a novel process for the production and purification of microbial collagenase (Microbial Collagenase EC 3.4.24.3) produced by the non-pathogenic aerobic bacteriumchemovar.(NCIMB Number: 1 1038, synonym LMG 3418, hereinafter called), which said process provides high production levels of collagenase with a stable, reproducible, cheap fermentation process. The collagenase produced fromaccording to the process described herein also presents a specific activity superior to that of other microbial collagenases, is stable in aqueous solution, and can be frozen without significant damage. A further subject of the present invention is pharmaceutical compositions containing collagenase obtained according to the production and purification process described, for the purpose of therapeutic treatment of disorders characterized by collagen accumulation or for the treatment of blemishes/imperfections that benefit from reducing local collagen accumulations.

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Abano Terme, Italy

Michele Caputo