Merck Sharp + Dohme Corp.

Cold Spring Harbor Laboratory

Merck + Co., Inc.

Michele A Cleary

Gregory J Hannon

Peter S Linsley

Stephen H Friend

Julja Burchard

Lee Lim

Patrick Paddison

Aimee L Jackson

Ernest M Coffey

Jill F Magnus

Scott William Lowe

Lin He

Xingyue He

Kristopher A Killian

Kristopher A Kilian

Lee P Lim

The present invention provides methods of making a population of nucleic acid molecules, wherein each nucleic acid molecule comprises a predetermined nucleic acid sequence, each of said methods comprising the steps of: (a) synthesizing, on a substrate, a population of nucleic acid molecules wherein: i) each synthesized nucleic acid molecule comprises a predetermined nucleic acid sequence; and ii) each synthesized nucleic acid molecule is localized to a defined area of said substrate; (b) harvesting said population of synthesized nucleic acid molecules from said substrate to yield harvested nucleic acid molecules; and (c) introducing said harvested nucleic acid molecules into vector molecules.

Method for making populations of defined nucleic acid molecules

In one aspect, the invention generally relates to use of miR-34 as a biomarker to estimate TP53 function in a cell. In another aspect, the invention generally relates to multiple uses of miR-34 and siRNAs functionally and structurally related to miR-34 for the treatment of cancer.

Methods of using MIR34 as a biomarker for TP53 functional status

In one aspect, the invention generally relates to compositions comprising miR-34 and siRNAs functionally and structurally related to miR-34 for the treatment of cancer.

Compositions comprising MIR34 therapeutic agents for treating cancer

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Jamison, PA, United States of America

Michele A Cleary