Laboratoire Francais Du Fractionnement Et Des Biotechnologies

Laboratoire Francais Du Fractionnement Et Des

Association Pour L'essor De La Transfusion Sanguine Dans

Michel Poulle

Sami Chtourou

Serge Martel

Miryana Burnouf (nee Radosevich)

The invention relates to a method for purifying factor B, comprising the steps consisting in:

Method for purifying factor B

This invention relates to a process for the preparation of a very high purity von Willebrand factor concentrate from a biological fraction containing von Willebrand factor, including a separation by anion exchange chromatography using a vinyl polymer support of weak base type, the separation comprising the steps of loading of the chromatographic support with the fraction containing von Willebrand factor, previously equilibrated with a suitable buffer, with a predetermined flowrate allowing the retention of the von Willebrand factor, washing of the support with an acidic buffer with a flowrate higher than the flowrate of the step a) until the not-retained proteins and the contaminants are removed, flushing and equilibrating of the chromatographic support with the buffer and using the flowrate of the step a), and elution of the von Willebrand factor by increasing of the ionic strength of the step c). The invention also relates to a von Willebrand factor concentrate for therapeutic use likely to be obtained by implementing the process wherein the rate of Factor VIII:C/FvW:RCo is less than 0.06%.

Process for the preparation of a von Willebrand (FvW) factor concentrate by chromatography and a FvW concentrate thus obtainable

Disclosed is a process for preparing a C1-esterase inhibitor concentrate, of human plasma origin, comprising 2 separations by chromatography on ion exchanger gels of tentacular type.

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Wavrin, France

Michel Poulle