Centre National De La Recherche Scientifique

Aventis Pharma S.a.

Agence Nationale De Valorisation De La Recherche (anvar)

Rhone-poulenc Rorer S.a.

Michel M Guerineau

Jean-Luc Pernodet

Henri Heslot

Karine Tuphile

Claude Gerbaud

Philippe Fournier

Juliette Hagege

Annick Friedmann

Alain Raynal

Guennady Sezonov

Michel Aigle

Pascal Simonet

Guennadi Sezonov

Tamara Smokvina

François Francou

Pascale Jeannin

Hughes Blanc

Maria Ball

Francois Lacroute

Sophie Courtois

Asa Frostegard

Camela Cappellano

Frederic Boccard

The invention concerns a method for preparing nucleic acids from an environment sample, more particularly a method for obtaining a library of nucleic acids from a sample. The invention also concerns nucleic acids of nucleic acid libraries obtained by said method their use in the synthesis of novel compounds, in particular novel compounds of therapeutic interest. The invent further concerns novel means used in the method for obtaining said nucleic acids, such as novel vectors and novel processes for preparing such vectors or recombinant host cells containing said nucleic acid. Finally, the invention concerns methods for detecting a nucleic acid of interest within a library of nucleic acids resulting from said method, and nucleic acids detected by said method and polypeptides encoded by said nucleic acids.

Method for obtaining nucleic acids from an environment sample, resulting nucleic acids and use in synthesis of novel compounds

Regulatory nucleic acid sequences and uses in actinomycetes

The present invention relates to a vector for cloning and expression of a DNA sequence coding for a particular protein in an Actynomycetes strain. The process comprises:

Cloning and expression vectors in an actinomycetes strain, process for

The present invention relates to a plasmid vector for cloning and expression of a protein in a microorganism, which comprises at least one structural gene which codes the synthesis of the said protein and elements which ensure the expression of the said structural gene in a microorganism, and wherein the promotion of the structural gene is ensured by the expression promotor of the .beta.-glucosidase gene in yeasts. The present invention also relates to microorganisms transformed by the said vectors, in particular a transformed strain of S. cerevisiae, a fermentation process using the said vectors, and the enzymes prepared by the said process, in particular .beta.-glucosidase.

Plasmid vectors for cloning and expression of a protein in a

A hybrid plasmid comprising at least the DNA of a bacterial plasmid, all or part of the DNA of the 2.mu. plasmid of yeast and a segment of DNA including the gene URA.sub.3.sup.+ of yeast. These plasmids are useful as vectors of exogenous DNA in yeasts.

Hybrid plasmids and microorganisms containing them

Growing community of inventors

Paris, France

Michel M Guerineau