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Centre National De La Recherche Scientifique

L'institut National De La Recherche Agronomique

Michel Kaczorek

Hassan Chaabihi

Martine Cerutti

Laurent Gauthier

Gerard Devauchelle

Marie-Alix Poul

Marie-Paule Lefranc

L&eacute;na Edelman

Christel Margaritte

Recombinant baculovirus used as an expression vector in the production of immunoglobulins with an insect cell. The invention is characterized by a first expression cassette comprising a first sequence coding for at least one portion of an immunoglobulin heavy chain, wherein the sequence is transcriptionally controlled by a first baculovirus promoter, and a second expression cassette comprising a second sequence coding for at least one portion of an immunoglobulin light chain, said second sequence being transcriptionally controlled by a second baculovirus promoter. The first and second promoters are two different promoters or derivatives of different promoters, the first and second promoters residing at different loci.

Recombinant baculovirus and use thereof in the production of monoclonal antibodies

Cloning of DNA fragments which code for the light chain or the heavy chain variable domain of the D7C2 monoclonal antibody within a baculovirus. The invention also concerns the expression of these DNA fragments in insect host cells, the anti-rhesus D recombinant monoclonal antibody so obtained and its use.

Monoclonal recombinant anti-rhesus D (D7C2) antibody

Growing community of inventors

Montferrier, France

Michel Kaczorek