National Research Council of Canada

Oxford University Innovation Limited

National Research of Canada

Michel Gilbert

Warren W Wakarchuk

N Martin Young

Scott Houliston

Michael Paul Jennings

Traian Sulea

Yves Durocher

Maria L Jaramillo

Edward Richard Moxon

Maurizio Acchione

Elizabeth Willis

Martin N Young

Stephane Bernatchez

The present invention relates to compounds, compositions, and methods are provided for covalently linking a cargo molecule, such as a therapeutic or a diagnostic agent, to a glycan in the Fab region of an antibody. Also provided are methods of modeling and producing antibodies having de novo Fab glycosylation sites. Also provided are antibody carrier conjugates, methods of using the conjugates.

Antibody glycoconjugates and methods of production and use

The invention relates to sialate-O-acetyltransferase (SOAT) polypeptides, nucleic acids that encode the polypeptides, and methods of using the polypeptides.

SOAT polypeptide reaction mixture

&#x3b1;-1,4-galactosyltransferase (CgtD) polypeptides, nucleic acids that encode the polypeptides, including a polypeptide fromstrain LIO87 have been isolated and characterized. A method of producing a galactosylated saccharide comprising contacting an acceptor saccharide containing a terminal galactose, a donor substrate comprising a galactose moiety and one of the CgtD polypeptides is described.

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The invention relates to poly-sialyltransferse polypeptides with enhanced solubility and activity and methods of using the poly-sialyltransferases for production of poly-sialylated end products, e.g., oligosaccharides, glycoproteins and glycolipids.

Engineered versions of polysialyltransferases with enhanced enzymatic properties

CgtB proteins with enhanced beta 1,3-galactosaminyltransferase activity, nucleic acids that encode the CgtB proteins and methods for use of the CgtB proteins.

Engineered versions of CgtB (&#x3b2;-1,3 galactosyltransferase) enzymes, with enhanced enzymatic properties

The invention relates to &#x3b2;-1,3-N-acetylgalactosaminyltransferase polypeptides, nucleic acids that encode the polypeptides, and methods of using the polypeptides.

The invention relates to a fusion protein comprising a bifunctional sialytransferase and a poly-sialytransferase and methods to use the fusion proteins for production of poly-sialylated end products, e.g. oligosaccharides and glycoproteins.

Self-priming polysialyltransferase

This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an &#x3b1;2,3- and an &#x3b1;2,8-activity. A &#x3b2;1,4-GalNAc transferase and a &#x3b2;1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example,species, including. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.

This invention provides prokaryotic &#x3b2;1,4-N-acetylgalactosaminyl (GalNAc) transferases involved in synthesis of lipooligosaccharide (LOS). &#x3b2;1,4-GalNAc transferases are obtained from, for example,species, including. In additional embodiments, the invention provides nucleic acids that encode the &#x3b2;1,4-GalNAc transferases, as well as expression vectors and host cells for expressing the &#x3b2;1,4-GalNAc transferases.

B1,4-N-acetylglucosaminyl (GALNAC) transferase polypeptides

This invention provides prokaryotic &#x3b2;1,4-N-acetylgucosaminyl (GalNAc) transferases involved in synthesis of lipooligosaccharide (LOS). The &#x3b2;1,4-GalNAc transferases can be obtained from, for example,species, including. In additional embodiments, the invention provides nucleic acids that encode the &#x3b2;1,4-GalNAc transferases, as well as expression vectors and host cells for expressing the &#x3b2;1,4-GalNAc transferases.

This invention provides fusion polypeptides that include a glycosyltransferase catalytic domain and a catalytic domain from an accessory enzyme that is involved in making a substrate for a glycosyltransferase reaction. Nucleic acids that encode the fusion polypeptides are also provided, as are host cells for expressing the fusion polypeptides of the invention.

Nucleic acid that encodes a fusion protein

This invention provides prokaryotic &#x3b2;1,4-N-acetylglucosaminyl (GalNAc) traosferases involved in synthesis of lipooligosaccharide (LOS). The &#x3b2;1,4-GalNAc transferases can be obtained from, for example,species, including. In additional embodiments, the invention provides nucleic acids that encode the &#x3b2;1,4-GaINAc transferases, as well as expression vectors and host cells for expressing the &#x3b2;1,4-GalNAc transferases.

The structure and specificity of a recombinant &#x3b1;2,3-sialyltransferase fromspp., is disclosed. Also provided are methods for using the &#x3b1;2,3-sialyltransferase in the production of desired carbohydrate structures and nucleic acids that encode the sialyltransferase.

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Hull, Canada

Michel Gilbert