Qiagen Sciences, LLC

Isoplexis Corporation

Michel Georges Perbost

Jerzy Olejnik

Thomas D Perroud

Luisa Andruzzi

Dona Hevroni

Austin Ricker

Timothy Pelletier

Minakshi Guha

Hsu-Yi Lee

Minakshi Gupta

The invention relates to methods, compositions, devices, systems and kits as described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis methods. In particular, the present invention contemplates the use of photoprotective mixture of compounds as imaging reagents to improve stability and storage of fluorescent compounds, including but not limited to, nucleotides with fluorescent labels.

Photoprotective mixtures as imaging reagents in sequencing-by-synthesis

The invention relates to methods, compositions, devices, systems and kits as described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis methods. In particular, the present invention contemplates the use of polyphenolic compounds, known as antioxidant additives, to improve the efficiency of Sequencing-By-Synthesis reactions. For example, gallic acid (GA) is shown herein to be one of many exemplary SBS polyphenolic additives.

Polyphenolic additives in sequencing by synthesis

Fiducial marking systems provided for forming a global coordinate system for a visual system having a limited field of view. The fiducial marking system includes a target surface that is movable relative to the visual system and a visual pattern associated with the target surface. The visual pattern includes a plurality of first lines and a plurality of second lines. Each first line is spaced by a first distance from each adjacent first line and each second line is spaced by a second distance from each adjacent second line, wherein the first distance is different than the second distance. Additionally, the first distance and the second distance are selected such that at least one first line and at least one second line are within the field of view at all relative positions of the visual system and the target surface.

Fiducial marking system

A flowcell for a sequencing instrument. The flowcell includes a fluid inlet, a fluid outlet, a flow channel formed between an at least partially transparent cover and a base and fluidly connecting the fluid inlet to the fluid outlet, and a capture substrate provided in the flow channel. The capture substrate includes microretainers configured to each receive a single microspot having a microspot diameter, and microretainer is separated from adjacent microretainers by an interstitial gap distance that is equal to or greater than the microspot diameter. A particle separator may be fluidly connected to the flowcell. The particle separator may include a microfluidic channel having an array of micropillars to transfer a plurality of the microspots to a loading buffer that may be delivered to the flowcell.

Flowcells with microretainers and particle separators for discrete seeding microspots

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Belmont, MA, United States of America

Michel Georges Perbost