Centre National De La Recherche Scientifique

Cis Bio International

Cisbio Bioassays

Centre National De La Recherche (Cnrs)

Cnrs and Fabrice Duprat

Ion Beam Applications S.a.

Michel Fink

Michel Lazdunski

Florian Lesage

Fabrice Duprat

Georges Romey

Eric Honore

Eric Trinquet

Jacques Barhanin

Eric Guillemare

Gérard Mathis

Hervé Bazin

Jurriaan Zwier

Iris Pribilla

Norbert Tinel

Hervé Ansanay

Stéphane Martinez

Robert Poole

Amanda J Patel

Delphine Jaga

Herve Ansanay

Francois Maingret

Hamed Mokrane

Sraboni Ghose

The invention relates to a method for detecting the binding of an antibody to an Fc receptor present on the surface of a cell as well as to a method for determining the level of glycosylation of an antibody. The invention also relates to a reagent kit for carrying out these methods.

Method for determining the glycosylation of an antibody

The instant invention provides for methods for detecting the internalization of a transmembrane protein of interest expressed at the surface of a cell. More specifically, the methods involve (a) labelling the protein of interest with a fluorescent metal complex, the lifetime of which is greater than 0.1 ms, (b) adding to the reaction medium a composition capable of causing the internalization of the protein of interest, (c) adding to the reaction medium (1) a modulating agent which is a fluorescent FRET acceptor compound compatible with the fluorescent metal complex, the final concentration of which in the reaction medium is greater than 10M; or (2) a reducing agent, the redox potential of which is less than +0.1 V and preferably between 0.25 and 0.75 V; or (3) an agent which binds specifically, by non-covalent bonding, with the fluorescent metal complex; (d) adding a metal ion which competes with the rare earth so as to form a non-fluorescent metal complex; (d) measuring the luminescence emitted by the reaction medium at the emission wavelength of the fluorescent metal complex and/or at the emission wavelength of the modulating compound when the compound is a fluorescent acceptor compound; and (e) comparing the signal measured in step d) with a reference signal measured on cells having been subjected only to steps a) and c).

Method for detecting membrane protein internalization

The invention relates to a quantitative non-microscopic method of detecting intracellular interactions between biomolecules in living cells, in response to a biological or pharmacological stimulation, by a time-resolved proximate energy transfer effect between two members of a fluorescence donor/acceptor pair.

Method for detecting intracellular interaction between biomolecules

A purified protein including a mechanosensitive potassium channel activated by at least one polyunsaturated fatty acid and riluzole and the use of channels in drug screening.

Mechanosensitive mammalian potassium channel activatable by polyunsaturated fatty acids

Human and mouse TREK-1 potassium transport proteins are disclosed as well a method for using potassium transport proteins for identifying substances having anesthetic properties, such as producing a safe, reversible state of unconsciousness with concurrent amnesia and analgesia in a mammal upon inhalation, in a method comprising (a) contacting the test substance with a mammalian potassium transport protein, wherein said potassium transport protein exhibits outward-going potassium rectification; and (b) determining the potassium transport activity of the potassium transport protein, wherein an activation of potassium transport is indicative of the test substance having anesthetic properties.

Method for the identification of anesthetics

This invention relates to the cloning of a member of a new potassium channel named TWIK-1. More specifically, it relates to an isolated and purified nucleic acid molecule coding for a protein constituting a potassium channel exhibiting the proper-ties and structure of the TWIK-1 type channel, as well as the protein coded by this nucleic acid molecule. The invention also relates to the use of this nucleic acid molecule to transform cells, and the use of these cells expressing the potassium channels exhibiting the properties and structure of the TWIK-1 type channel for the screening of drugs.

Isolated TWIK-1 potassium channel proteins

A purified protein comprising a mechanosensitive potassium channel activated by at least one polyunsaturated fatty acid and riluzole and the use of such channels in drug screening.

Method for screening substances capable of modulating the activity of a TRAAK potassium channel

This invention relates to a new member of a recently recognized TWIK potassium,channel family, herein identified as TASK, for TWIK-related acid-sensitive K,channel. This is the first cloned mammalian channel that produces K,currents that possesses all the characteristics of background conductances. The invention also relates to various constructs including the TASK or related human potassium channel family, and their uses.

Family of mammalian potassium channels, their cloning and their use, especially for the screening of drugs

The present invention relates to the cloning of a member of a new potassium channel named TWIK-1. More specifically, it relates to an isolated and purified nucleic acid molecule coding for a protein constituting a potassium channel exhibiting the properties and structure of the TWIK-1 type channel, as well as the protein coded by this nucleic acid molecule. The invention also relates to the use of this nucleic acid molecule to transform cells, and the use of these cells expressing the potassium channels exhibiting the properties and structure of the TWIK-1 type channel for the screening of drugs.

Family of mammalian potassium channels, their cloning and their use

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Bagnols sur Ceze, France

Michel Fink