Centre National De La Recherche Scientifique

Université´ D'aix-marseille

Institut National De La Sante Et De La Recherche Medicale

Biomerieux, Inc.

Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Université De La Méditerranée - Aix-marseille II

Fondation Mediterranee Infection

Fondation Mediterranee Infection Faculté De Médecine

Universite De La Mediterranee (aix-marsille Ii)

Inserm (institut National De La Sante Et De La Recherche Medicale)

Assistance Publique Hopitaux De Marseille

Université De La Méditerranée

Inserm

Pocramé

Michel Drancourt

Didier Raoult

Saber Khelaifia

Cedric Allier

Cédric Allier

Bernard La Scola

Sonia Chatellier

Marc Abdul Samad

Pierre-Yves Levy

Shady Asmar

Zoheira Djelouadji

Elodie Neau

The present invention relates to a method for cultivating in vitro in an acellular culture medium, bacteria for which the growth is sensitive to oxygen content, said bacterium being selected from anaerobic bacteria and intracellular micro-aerophilic bacteria, characterized in that uric acid is added into said acellular culture medium, and said bacterium is cultivated in said culture medium in the presence of oxygen.

Use of uric acid for culturing bacteria sensitive to oxygen tension

The present invention relates to the biological production of methane (Biogas) by co-culture in an aerobic atmosphere of a methanogenic bacterium and of an anaerobic bacterium capable of producing hydrogen, in a culture medium comprising or being supplemented with carbohydrate compound(s), notably starch and/or sugars, and supplemented with antioxidant compound(s).

Method for producing methane by means of aerobic co-culture of anaerobic micro-organisms

This method for detecting at least one particle in a bodily fluid is carried out via a detection system including a light source, a transparent substrate and a photodetector array, the substrate being positioned between the light source and the photodetector. This method includes the placement of a droplet of bodily fluid on the substrate, the illumination of the droplet via the light source, the acquisition of several successive images of the droplet via the photodetector, each image being formed by radiation transmitted by the illuminated droplet and including at least one elementary diffraction pattern, each elementary diffraction pattern corresponding to waves diffracted by a particle upon illumination of the droplet, the identification, via the acquired images of the mobile elementary diffraction patterns, and the counting of moving particles in the droplet, via the identified mobile elementary diffraction patterns.

Method and system for detecting at least one particle in a bodily fluid, and associated method for diagnosing meningitis

The present invention relates to a novel mycobacteria culture medium, particularly for mycobacteria of thecomplex, that significantly reduces the culture isolation time and thus the time for diagnosing the mycobacteria, particularly those for tuberculosis. A culture medium according to the invention contains defibrinated blood, lecithin, and decomplemented fetal calf serum. The present invention also relates to a culture method and to a method for identifying mycobacteria, particularly bacteria of thecomplex. The present invention also relates to a novel decontamination method through chlorhexidine treatment of biological samples in an isolation and mycobacteria culture medium, and to a method for determining by phenotype the sensitivity of mycobacteria to antibiotics by means of a solid culture medium according to the invention. Finally, the present invention also relates to a method of identification in liquid phase by mass spectrometry which reduces time for diagnosing mycobacteriosis, particularly for tuberculosis.

Mycobacteria culture medium and method including mycobacteria of mycobacterium tuberculosis complex

The invention relates to a method of detecting, by means of molecular identification, a bacterium from one of the-type species. The inventive method is characterised in that the following are used: a fragment of the rpoB gene of said bacterium, comprising a nucleotide sequence selected from one of the SEQ. ID. No. 11 to 39 sequences, the reverse sequences and the complementary sequences; or an oligonucleotide comprising a sequence having at least 12 consecutive nucleotide patterns included in one of the SEQ. ID. No. 7 to 10 sequences, in which N represents a nucleotide selected from inosine and an equimolar mixture of 4 different nucleotides selected from A, T, C or G and from the oligonucleotides of the reverse sequences and complementary sequences.

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The invention concerns a method for detecting by molecular identification a bacterium of one of the species of genusand four related generaandwhich consists of using as probe or primer:

The invention concerns the total sequence of the Tropheryma whippelii bacteria rpoB gene, characterized in that it consists of the nucleotide sequence SEQ ID no. &#xb0;9, and the nucleotide sequences derived from said sequence specific of thebacteria and their use as probe and primer.

Sequence of the tropheryma whippelii bacteria rpoB gene and oligonucleotide for molecular diagnosis of whipple's disease

The invention concerns single stranded oligonucleotides comprising a sequence of at least 12 consecutive nucleotide motifs contained in one of the sequences SEQ ID NO: 1 to SEQ ID NO: 4 wherein 'n' represents an identical or different nucleotide selected among inosine or an equimolar mixture of 4 different nucleotides selected among a, t, c or g, and among oligonucleotides complementary thereto. The invention also concerns using the oligonucleotides to detect bacteria of the Spirochaetales order. The invention is based on the use of specifically defined sequences in the rpoB gene of spirochetes coding for the beta subunit of the bacterial RNA polymerase.

Single stranded oligonucleotides, probes, primers and method for detecting spirochetes

Growing community of inventors

Marseilles, France

Michel Drancourt