Centre National De La Recherche Scientifique

Institut Curie

Cytoo

Michel Bornens

Manuel Thery

Matthieu Piel

Alexandra Fuchs

Yoran Margaron

Sebastien Degot

Mathieu Fernandes

Joanne Young

Jie Liu

Delphine Morales

Maria Luisa Calvo Munoz

Aurelie Berthelot

A method and device for standardizing myoblast differentiation into myotubes, including a substrate () and at least one cell-adhesive pattern () for culturing myoblasts on the substrate. The pattern () has an elongated surface. A central region (C) and two lateral regions (L) extend from the central region in both directions along a longitudinal axis of the pattern. The ratio between the maximum width (W) of the central region (C) and the maximum width (W) of the lateral regions (L) is greater than or equal to 2. The ratio between the length (L) and the maximum width (W) of the pattern () is less than or equal to 4. The method includes providing a device as described above, depositing myoblasts on at least one cell-adhesive pattern of the device, culturing the myoblasts in a differentiation medium to promote cell differentiation into myotubes and constrain elongation of the myotubes.

Device and method for standardizing myoblast differentiation into myotubes

The present invention relates to methods and devices for adhering cells in a specific and predetermined position with an adhesive control of internal cell organization, methods for preparing such devices, methods for studying modifications of cell shape and global internal cell organization such as the distribution of cellular compartments, centrosome centering, spindle orientation, internal compartmentalization and internal transports, methods for screening compounds of interest which enhance or inhibit specific cell functions.

Methods and device for adhesive control of internal cell organisation

Growing community of inventors

Sceaux, France

Michel Bornens