Biomerieux, Inc.

Michel Arnaud

Colette Jolivet-Reynaud

Guy Serre

Michel C Jolivet

Yasemin Ataman-Onal

Mireille Sebbag

Pascal T Dalbon

Christian Vincent

Michel Simon

Franck Berthier

Jacques Passagot

Jacqueline Dupret-Carruel

Elisabeth Girbal-Neuhauser

Jacqueline Dupret-carruel

A method for detecting an immune cellular response including the steps of: (a) suctioning a defined amount of the sample by an automated suction/discharge device, (b) discharging this amount, using the device, into container R, containing, beforehand, at least one stimulant for secretion of the molecule by the at least one cell, or else not containing such a stimulant, (c) when the container does not contain the stimulant beforehand, introducing into container R the at least one secretion stimulant contained in container E, using the device, and (d) allowing the sample and stimulant to incubate, forming a mixture, by the at least one cell (i) in container R or (ii) in another container S after suctioning and discharge of the mixture using the device, and (e) detecting the immune cellular response, the detection of the immune cellular response indicates the presence of an immune cellular response specific for the stimulant.

Method for detecting an immune cellular response

The invention concerns an artificial antigen specifically identified by the anti-filaggrin autoantibodies present in the serum of patients suffering from rheumatoid polyarthritis, and consisting of one polypeptide comprising all or part of the sequence of one filaggrin unit or of a related molecule, in which an arginine radical has been substituted by a citrulline radical. The invention also concerns the use of this antigen for diagnosing rheumatoid polyarthritis.

Antigens derived from filaggrin and their use for diagnosing rheumatoid polyarthritis

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Villeurbane, France

Michel Arnaud