Nederlandse Organisatie Voor Toegepast-natuurwetenschappelijk Onderzoek

Nederlandse Organisatie Voor Toegepast

Leids Universitair Medisch Centrum

Natuurwetenschappelijk Onderzoek Tno Leids Universitair Medisch Centrum

Nederlandse Organisatie Voor Toegepast-natuurwetenschappelijk Onderzoek Tno

Michaela Diamant

Johan Hendrikus Verheijen

Jan Roeland Occo Hanemaaijer

The present invention relates to a method for determining if a subject having Type II diabetes has a kidney disorder by measuring the level of matrix metalloproteinase 8 (MMP-8) in the urine of a subject having Type II diabetes and comparing this level to a reference sample or a sample from a healthy subject and determining if the subject has a kidney disorder base on the presence of increased levels of MMP-8 in the urine compared to the reference values or reference sample.

Method for determining if a subject having type II diabetes has a kidney disorder

A method for determining whether a urine sample has been obtained from a subject suffering from a kidney disorder involving kidney damage. The method is based on determining the level of a proteolytic enzyme in urine from the subject. Sample and optionally comparing this level with a reference value. The proteolytic enzyme preferably is a matrix metalloproteases, in particular MMP-2 or MMP-9. The method is particularly suitable for subjects with a condition or disorder that is associated with an increased risk of kidney disorders, such as diabetes. The method may adavnatageously be applied for early detection of kidney damage, in particular when the subject does not yet show any sign of microalbuminuria. The method may also be applied to a subject undergoing dialysis for determining whether the subject is suffering from remodelling of the peritoneal membrane. Thus, the method is particularly suited for patients undergoing continuous ambulatory peritoneal dialysis.

Proteolytic enzymes in urine as diagnostic parameters in diseases involving matrix remodelling

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GW Muiderberg, Netherlands

Michaela Diamant