Leica Mikrosysteme Gmbh

Leica Microsysteme Gmbh

Michael Zimmermann

Reinhard Lihl

Anton Lang

Rainer Wogritsch

Guenther Bock

Paul Wurzinger

Heinz Plank

Siegfried Tanki

Hubert Goll

Andreas Hallady

Ian Lamswood

Microtome

A microtome system has a cryomicrotome with a sectioning device in which is provided a preparation holder and a knife edge that are guided past one another inside a working space during a sectioning operation, in order to produce thin sections of a preparation retained in the preparation holder. A micromanipulator is operable outside the working space. With the micromanipulator, a tool for retention of a specimen support is positioned proximate the knife edge during a sectioning operation in order to receive the sections that are produced, preferably for substantially stationary retention of the specimen support.

Micromanipulator for a cryomicrotome

A freeze fracture machine () comprises a vacuum chamber (); a sample receptacle () for receiving a sample to be processed and a knife receptacle () for receiving a knife () for processing of the sample arranged in the vacuum chamber (); a transfer lock () on the vacuum chamber () through which a sample and a knife () are introducible into the vacuum chamber () and positionable in the vacuum chamber () by means of a positioning apparatus (); the sample and the sample receptacle () being positionable with respect to one another such that the sample is mountable on the sample receptacle () by means of the positioning apparatus (); and the knife () and the knife receptacle () being positionable with respect to one another such that the knife () is mountable on the knife receptacle () by means of the positioning apparatus ().

Freeze fracture machine

The present invention relates to a cryopreparation chamber () for preparing and manipulating a sample for electron microscopy, the cryopreparation chamber () being cooled by a primary cryogen, the cryopreparation chamber () including a first and a second chamber portion (), the second chamber portion () being detachably placeable () on the first chamber portion (), and moreover, the second chamber portion () being provided, in its outer wall (), with an access port () through which a specimen holder () for an electron microscope can be inserted into the cryopreparation chamber (). The present invention also relates to a cryopreparation device () which is suitable for cryopreparing a sample for an electron microscope and includes such a cryopreparation chamber ().

Cryopreparation chamber for manipulating a sample for electron microscopy

An apparatus () for processing specimens (), comprising an observation device (), a specimen holder () for receiving the specimen () to be processed, and a tool holder (), the tool holder () being pivotable in a plane normal to its longitudinal axis (L') by a pivot arm (), and being rotatable about its longitudinal axis (L&#x2032;); furthermore a drive system for selectable execution of the pivoting of the pivot arm () is provided.

Apparatus for processing a specimen

A knife holder for a microtome having a vibrating knife comprises a support element and a clamping element movable relative to the support element. The support element is embodied with a support surface for the knife, and the clamping element is embodied with a clamping surface. For clamping the knife, the support surface and clamping surface are settable at a distance of 0.1 to 1.5 from one another.

Knife holder for a microtome having a vibrating knife

A Dewar vessel () for automated cryosubstitution or low-temperature substitution is disclosed. The invention further discloses an apparatus () for automated liquid transfer for cryosubstitution or low-temperature substitution. The apparatus () encompasses a container () that encompasses at least one specimen holder () and at least one reservoir holder (); and wherein a movable transfer container () for automated exchange of at least one liquid () between the at least one specimen holder () and the at least one reservoir holder () is provided.

Dewar vessel for automated cryosubstitution or low-temperature substitution, and apparatus for automated cryosubstitution or low-temperature substitution

An apparatus () for tissue preparation for the tissue embedding, having a transport plate () defining a circumferential edge (). At the circumferential edge () several recesses () are formed, each of which comprises a plurality of several circular cut outs () being arranged so that it has an opening into the direction of the circumferential edge (). The transport plate () is surrounded by a housing () which defines an inner upper side () on which a heating and cooling device () is provided.

Apparatus for tissue processing for the tissue embedding and mounting for the processing containers for tissue processing

An apparatus () for tissue preparation for the tissue embedding, having a transport plate () that is surrounded by a housing (). The housing defines an inner upper side (), a heating and cooling device (), that is provided on the inner upper side () of the housing () and a mechanic module () for turning the transport plate () and for raising and lowering the transport plate (). The mechanic module () comprises a first motor () exclusively for turning the transport plate () around an axle () and a second motor () exclusively for raising and lowering the transport plate () in the direction of the axle ().

Apparatus for tissue processing for the tissue embedding

A cooling chamberfor a microtomeis disclosed. A knifehaving a cutting edgeis arranged in the cooling chamberopposite a sample holderhaving a sampleretained therein. The microtomefurther possesses a stereomicroscopehaving an optical system, the optical systemdefining an optical axis. The region of the sampleand of the cutting edgeof the knifeis observable with the stereomicroscope. There is mounted in the cooling chamberan illumination systemthat emits lightand is directed onto a surfaceof the knifein such a way that the lightreflects from the surfaceparallel to the optical axis

Cooling chamber and system of a cooling chamber with a microtome

A container () for tissue preparation for the tissue embedding, having a base body (), that is formed out cylindrical shaped side wall (), a floor () and several bridges (). The bridges () subdivide the container () in several sub-containers (). The side wall () has a plurality of slit shaped openings (), that extend essentially over the total height of the side wall ().

Growing community of inventors

Leopoldsdorf, Austria

Michael Zimmermann