Emulate, Inc.

Cedars-sinai Medical Center

Cincinnati Children's Hospital Medical Center

Michael Workman

Clive N Svendsen

Robert N Barrett

Dhruv Sareen

Uthra Rajamani

Daniel Levner

Christopher David Hinojosa

Norman Wen

Geraldine A Hamilton

S Jordan Kerns

Jacob Fraser

Magdalena Kasendra

Carol Lucchesi

Jefferson Puerta

Stephen R Targan

Stephan R Targan

Chris Hinojosa

James Macormack Wells

Jorge Orlando Munera

Michael A Helmrath

Carey Lane Watson

Maxime Mickael Mahe

Jordan Kerns

Magdelena Kasendra

Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.

Systems and methods for growth of intestinal cells in microfluidic devices

Disclosed are methods for making a vascularized hollow organ derived from human intestinal organoid (HIOs). The HIOs may be obtained from human embryonic stem cells (ESC's) and/or induced pluripotent stem cells (iPSCs), such that the HIO forms mature intestinal tissue. Also disclosed are methods for making a human intestinal tissue containing a functional enteric nervous system (ENS).

Method of making in vivo human small intestine organoids from pluripotent stem cells

Growing community of inventors

Santa Monica, CA, United States of America

Michael Workman