Sartorius Stedim Biotech Gmbh

Bavarian Nordic A/s

Otto-Von-Guericke-Universität

Max-Planck-Gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Michael Wolff

Udo Reichl

Rene Faber

Sara Post Hansen

Anders Peter Gram

Lars Opitz

Pavel Marichal-Gallardo

Michael Martin Pieler

In a first aspect, the present invention relates to a method for the purification of virus compositions as well as biological macromolecular compounds in a sample comprising mixing the sample with osmolytes, like non-ionic organic polymers and contacting the mixed sample with a hydrophilic membrane, optionally washing the membrane, and eluting the virus preparations or biological macromolecular components from the membrane with an eluting solution containing reduced amounts or no osmolytes, like non-ionic organic polymer. Moreover, virus compositions and biological macromolecular components obtainable with the method according to the present invention are provided as well as the use of the method according to the present invention for purification of virus compositions including whole virus particles and virus-like particles or biological macromolecular components.

Method for the separation of virus compositions including depletion and purification thereof

The present invention relates to methods for purification of Vaccinia viruses (VV) and/or Vaccinia virus (VV) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production.

Purification of vaccinia viruses using hydrophobic interaction chromatography

The present invention relates to methods for the preparation of functionalized sulfated cellulose membranes. In particular, the present invention relates to the preparation of sulfated cellulose membranes under specific reaction conditions allowing to provide a sulfated cellulose membrane useful for pseudo-affinity purification. In another aspect, the present invention relates to the sulfated cellulose membrane itself as well as its use for isolation of proteinaceous compositions. Finally, the present invention provides a method for isolating whole virus, virus proteins or heparin binding molecules comprising the step of affinity purification using the sulfated cellulose membrane according to the present invention.

Method for the preparation of sulfated cellulose membranes and sulfated cellulose membranes

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Biederitz, Germany

Michael Wolff