Phytera Incorporated

Michael William Fowler

Debbie Grey

Gagik Stephan-Sarkissian

A process for the production of an extracellular peroxidase using confectionery waste is disclosed. The first step of the process requires culturing a piece of plant tissue containing extracellular peroxidase-producing cells from a plant of the genus Acer, more specifically Acer pseudoplantanus. The culture medium is a solid culture medium and the culturing step is carried out until a callus forms on the solid culture medium. Further, the plant cells produced in the callus are dispersed into a liquid culture medium to form a suspension of plant cell culture. The suspension culture medium contains confectionery waste products which provide 1 to 15% by weight of sugars (i.e. fructose, glucose and sucrose). The culturing of the plant cells in suspension in the liquid culture medium with the concomitant accumulation of the extracellular peroxidase in the liquid culture medium and separating the enzyme therefrom. Also disclosed is a nutrient medium for the plant cell suspension which contains confectionery waste products which provide 1 to 15% by weight of sugars and a concentration of phytohormones is also contained within the nutrient medium for the plant cell suspension.

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Nr. Matlock, United Kingdom

Michael William Fowler