University of Georgia Research Foundation, Inc.

North Carolina State University

Michael W W Adams

Robert M Kelly

Aaron B Hawkins

Gina Lynette Pries Lipscomb

Gerrit Jan Schut

Angeli Lal Menon

Janet Westpheling

Francis E Jenney, Jr

Joel Andrew Farkas

Junsong Sun

Gina L Lipscomb

Robert C Hopkins

Provided herein are genetically engineered microbes that include at least a portion of a carbon fixation pathway, and in one embodiment, use molecular hydrogen to drive carbon dioxide fixation. In one embodiment, the genetically engineered microbe is modified to convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof at levels greater than a control microbe. Other products may also be produced. Also provided herein are cell free compositions that convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof. Also provided herein are methods of using the genetically engineered microbes and the cell free compositions.

Sequestration of carbon dioxide with hydrogen to useful products

Provided herein are polypeptides having hydrogenase activity. The polypeptide may be multimeric, and may have hydrogenase activity of at least 0.05 micromoles Hproduced minmg protein. Also provided herein are polynucleotides encoding the polypeptides, genetically modified microbes that include polynucleotides encoding one or more subunits of the multimeric polypeptide, and methods for making and using the polypeptides.

Hydrogenase polypeptide and methods of use

Provided herein are methods for transforming awith a polynucleotide. In one embodiment, the method includes contacting awith a polynucleotide under conditions suitable for uptake of the polynucleotide by the, and identifying transformants at a frequency of, for instance, at least 10transformants per microgram DNA. Also provided are isolatedhaving the characteristics ofCOM1, and plasmids that include an origin of replication that functions in a. The plasmid is stable in a recipientwithout selection for more than 100 generations and is structurally unchanged after replication infor more than 100 generations.

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Growing community of inventors

Athens, GA, United States of America

Michael W W Adams