Monsanto Technology LLC

Michael W Petersen

Xudong Ye

Kevin Lee Deppermann

Larry A Gilbertson

Brian J Martinell

Jaishree M Chittoor

Mohammed Oufattole

Shihshieh Huang

David V Butruille

Sam R Eathington

John Tamulonis

Bruce J Schnicker

Heather M Forbes

Stanton B Dotson

Edward James Williams

Yuechun Wan

David A Somers

David A Walters

Paul S Chomet

Qi Wang

Susan Jane Johnson

Amy J Miyamoto

Amy M Nichols

Stanislaw Flasinski

Kenneth A Barton

Yong Huang

Kenneth John Piller

Toni Armstrong

Laura J Crow

Wei Wu

Kim A Beazley

Bei Zhang

Allen N Ondes

David W Finley

Michael D Edgerton

William M Fischer

Jill Deikman

John M Jensen

David N Walters

Allen T Christian

Terri B Hinchey

Susan Johnson

David Walters

James J Schaefer

Chad Allen Stendal

Qiang Zhang

Heather Forbes

Xudong Xe

Shinhshieh Huang

Kenneth Barton

The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

Methods for plant transformation using spectinomycin selection

A method is provided for use in analyzing seeds, in a population of seeds, for one or more desired characteristic. The method includes removing tissue from individual seeds in a population of seeds using an automated seed sampler, while preserving germination viability of the seeds, and analyzing the tissue for the presence or absence of one or more desired characteristic. The method then includes, based on the analysis of the tissue, quantifying the one or more desired characteristic for each of the individual seeds that possess the one or more desired characteristic.

Methods of seed breeding using high throughput nondestructive seed sampling

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

Plant regulatory elements and uses thereof

The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

DNA constructs for obtaining marker-free transgenic plants

An automated method for sampling seeds generally includes removing material from a seed at an automated sampling station and then, after removing the material from the seed, detecting, by at least one sensor, movement of the seed out of the automated sampling station. The automated method may also include, after removing the material from the seed, transferring the seed from the automated sampling station to a seed container and transferring the material removed from the seed from the automated sampling station to a sample container.

Automated seed sampler and methods of sampling, testing and bulking seeds

Recombinant DNA molecules and constructs useful for modulating gene expression in plants, including molecules derived fromsequences, are provided. Plants, plant cells, plant parts, and seeds comprising recombinant DNA molecules operably linked to heterologous transcribable DNA molecules are further provided, as are methods of their use.

Plant regulatory elements derived from medicago truncatula 3'UTR sequences, and uses thereof

A method is provided for use in a plant breeding program to generate a population having one or more desired traits. The method includes removing a tissue sample from each of one or more seeds using an automated seed sampler while preserving germination viability of the sampled seeds; analyzing the tissue samples for the presence or absence of a genetic sequence associated with at least one desired trait; based on the analysis of the tissue samples, cultivating plants from the sampled seeds that either possess or lack the genetic sequence associated with the at least one desired trait; creating a parental cross using at least one of the cultivated plants; and selecting seeds from the parental cross and cultivating offspring of the parental cross using the selected seeds.

Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.

Vectors and methods for improved plant transformation efficiency

A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.

Automated systems for removing tissue samples from seeds, and related methods

Recombinant DNA molecules and constructs useful for modulating gene expression in plants, including molecules derived fromsequences, are provided. plants, plant cells, plant parts, and seeds comprising recombinant DNA molecules operably linked to heterologous transcribable DNA molecules are further provided, as are methods of their use.

Growing community of inventors

Sauk City, WI, United States of America

Michael W Petersen