Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Calgene Corporation

Other

University of California

Monsanto Technology LLC

Corteva Agriscience LLC

Dsm IP Assets B.v.

Maxygen, Inc.

Martek Biosciences Corporation

Lifeedit Therapeutics, Inc.

Maxyag, Inc.

Michael W Lassner

James George Metz

Saverio Carl Falco

Andrew Mark Cigan

Zhongsen Li

Kathryn Dennis Lardizabal

Kevin E McBride

Jack Q Wilkinson

Zhan-Bin Liu

Brian McGonigle

Loren Lee Looger

Huirong Gao

Albert Laurence Lu

James Jefferson Smith

Derek Jantz

James Kevin Presnail

Rafael Herrmann

Christopher Jay Scelonge

Diane M Ruezinsky

Phillip A Patten

Marc C Albertsen

Yongzhong Wu

Howard P Hershey

Timothy A Mitsky

Jun-Zhi Wei

Leszek Aleksander Lyznik

Huw Maelor Davies

Daniel Siehl

Deborah J Hawkins

David Richard Corbin

Anthony John Kinney

Howard Glenn Damude

Janet Ann Rice

Ganesh M Kishore

Mark R Moore

David J Peterson

Rodolphe Barrangou

Mark J Nelson

Steven Henry Bass

Theodore Mitchell Klein

Balasulojini Karunanandaa

Tedd D Elich

Steven Gutteridge

Sergei Svitashev

Gusui Wu

Mathias L Muller

Robert Keenan

Hana S Ali

Daniel Facciotti

John I Yoder

James D Weiss

Martha Ann Post-Beittenmiller

Mark Nelson

Beth Savidge

Jian Lu

Zhan-bin Liu

Alison Van Eenennaam

Yuxia Dong

Mark Edward Nelson

Tyson D Bowen

Sean M Bertain

Mylavarapu Venkatramesh

Jun-zhi Wei

Janet A Rice

Emily Ng

Alexandra Briner Crawley

Sekhar S Boddupalli

Janet Nelsen

Michael R Pollard

Ajith Anand

Shaukat H Rangwala

Hyeon-Je Cho

York Moy

Mark Cigan

Carl Falco

B Ganesh Bhat

Barbara Ann Marie Rosen

Daniel L Siehl

Janet Nelson

Robert J Grebenok

Vesna Djukanovic

Spencer Charles Jones

Gowri Shah

Steven delCardayre

Robert J Keenan

Deborah Hawkins

Kevin E Mcbride

Barabara Ann Marie Rosen

Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell, without incorporating a selectable transgene marker. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed, without incorporating a selectable transgene marker. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a guide polynucleotide/Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell, without incorporating a selectable transgene marker.

Methods for producing genetic modifications in a plant genome without incorporating a selectable transgene marker, and compositions thereof

Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.

Methods for producing a complex transgenic trait locus

Compositions and methods for binding to a target sequence of interest are provided. Compositions include fusion proteins between DNA binding proteins or protein domains and nucleic acid modifying proteins or protein domains. The compositions find use in cleaving or modifying a target sequence of interest, visualization of a target sequence of interest, and modifying the expression of a sequence of interest. Compositions comprise RNA-guided nuclease polypeptides, CRISPR RNAs, trans-activating CRISPR RNAs, guide RNAs, deaminases, and nucleic acid molecules encoding the same. Vectors and host cells comprising the nucleic acid molecules are also provided. Further provided are CRISPR systems for binding a target sequence of interest, wherein the CRISPR system comprises an RNA-guided nuclease polypeptide and one or more guide RNAs. Also provided are deaminases which may be fused to a DNA-binding polypeptide and may be useful for gene editing.

Polypeptides useful for gene editing and methods of use

Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and active fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or active fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part.

Compositions and methods comprising male fertility sequences

Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.

Methods and compositions for generating complex trait loci

Compositions and methods comprising polynucleotides and polypeptides having EPSP (5-enolpyruvylshikimate-3-phosphate) synthase (EPSPS) activity are provided. In specific embodiments, the sequence has an improved property, such as, but not limited to, improved catalytic capacity in the presence of the inhibitor, glyphosate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the EPSPS sequences. Various methods of employing the EPSPS sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

Plant EPSP synthases and methods of use

Methods and compositions for-mediated transformation of plants are provided. Methods include but are not limited to using anstrain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include anstrain, transfer DNAs, constructs and/or plasmids. These includestrains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.

Ochrobactrum-mediated transformation of plants

The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells, plants explants, seeds and grain.

Compositions and methods for producing plants resistant to glyphosate herbicide

Growing community of inventors

Foster City, CA, United States of America

Michael W Lassner