Case Western Reserve University

The United States of America, As Represented by the Secretary, Department of Health and Human Services

Vanderbilt University

University Hospitals Cleveland Medical Center

The Cleveland Clinic Foundation

U.S. Government Nih Division of Extramural Inventions and Technology Resources (deitr)

University of Pittsburgh

Michael W Jenkins

Andrew M Rollins

Stephen J Lewis

Hillel Judah Chiel

William J Dupps

E Duco Jansen

Yehe Liu

Osman Chughtai

Emilie Lothet

Eric Duco Jansen

Matthew R Ford

James M Seckler

Gjinovefa Kola

Brecken Blackburn

Shi Gu

B J Dupps

Junqi Zhuo

Junqi Zhou

Kendrick M Shaw

Emilie H Lothet

Charles Horn

Described herein are methods for imaging a fluorescent bioassay (including a substrate, such as dispersed cellular sample, exposed to one or more exogenous fluorophore and/or fluorescent probe that accumulate in a structure of interest). The bioassay can be excited with Type-C ultraviolet (UVC) light produced by one or more light emitting diode (LED). The UVC can have a center wavelength that causes emission by the fluorescent bioassay. A digital optical device can collect a signal emitted from the fluorescent bioassay in response to the excitation. The methods relate in particular to Microscopy with Ultraviolet Surface Excitation (MUSE) imaging.

Methods for microscopy with ultraviolet surface excitation (MUSE) imaging

Described herein are systems and methods for improving light-sheet microscopy with cost-effective and simplified components. Such cost-effective and simplified components can be implemented in a light focusing system, a light generation system, and/or in imaging system. The light focusing system can be improved by attaching a voice coil motor to a focusing lens to increase the imagable field of view. The light generation system can be improved with a multimode laser diode to increase the uniformity of the beam profile and to increase the usable optical power. The imaging system can be improved by using a fluid chamber with positive cylindrical optical window for minimizing spherical aberrations.

Systems and methods for improved light-sheet microscopy

The present disclosure relates to an optical clearing method called lipid-preserving index matching for prolonged imaging depth (LIMPID). The optical clearing method can include simply immersing a sample in an optical clearing solution before imaging (e.g., with microscopy or optical coherence tomography). The optical clearing solution can include water; an iodine-containing non-ionic radiocontrast agent; and urea.

Lipid-preserving refractive index matching for prolonged imaging depth for transparent tissue sample and composition

A method of stimulating ventilatory and/or respiratory drive in a subject in need thereof includes administering to the subject a therapeutically effective amount of a composition comprising an glutathione alkyl ester, adduct thereof, or pharmaceutically acceptable salt, tautomer, or solvate thereof.

Compositions and methods for stimulating ventilatory and/or respiratory drive

The present disclosure relates to a simple, fast, and low cost method for 3D microvascular imaging, termed 'scatter labeled imaging of microvasculature in excised tissue' (SLIME). The method can include perfusing a contrast agent through vasculature of a tissue sample. The contrast agent can include colloids and a dispersant. After the contrast agent is perfused through the vasculature, the vasculature of the tissue sample can be treated with a molecule that cross links with at least a portion of the dispersant to form a sticky, non-Newtonian polymer that prevents leakage of the contrast agent out of the vasculature of the tissue sample. The tissue sample can then be immersed in a solution comprising a clearing agent and subsequently imaged.

Scatter labeled imaging of microvasculature in excised tissue (SLIME)

The present disclosure relates to modulating neural activity within brainstem circuitry to evoke a certain bodily function (e.g., cardiorespiratory response) in a patient. A controller can configure a light signal (such as an infrared light signal) to deliver an amount of radiant exposure to a specific portion of the brainstem for a time. The specific portion of the brainstem can include brainstem circuitry that is the control center for the certain bodily function. The configured light signal can be delivered to the specific portion of the brainstem by an optrode for the time. During application, the light signal can deliver the amount of radiant exposure to the specific portion of the brainstem to evoke the certain bodily function.

Modulating neural activity in brainstem circuitry with light

The present disclosure relates to a simple, fast, and low cost method for 3D microvascular imaging, termed 'scatter labeled imaging of microvasculature in excised tissue' (SLIME). The method can include perfusing a contrast agent through vasculature of a tissue sample with a contrast perfusing unit (). The contrast agent can include colloids and a dispersant. After the contrast agent is perfused through the vasculature, the vasculature of the tissue sample can be treated with a cross-linking agent delivery unit () providing a molecule that cross links with at least a portion of the dispersant to form a sticky, non-Newtonian polymer that prevents leakage of the contrast agent out of the vasculature of the tissue sample. The tissue sample can then be immersed in a solution comprising a clearing agent with an optical clearing unit () and subsequently imaged.

One example includes a scanning system. The system includes a phase-decorrelation optical coherence tomography (PhD-OCT) scanner configured to implement a scanning sequence via an optical signal on a sample medium. The system also includes a scanning controller configured to provide control signals to the PhD-OCT scanner to implement the scanning sequence and to obtain phase and amplitude information associated with a reflected version of the optical signal. The phase and amplitude information can be indicative of microscale dynamics of the sample medium. The scanning controller can also implement an algorithm to calculate a quantitative parameter of the sample medium based on the phase and amplitude information associated with the sample medium.

Phase-decorrelation optical coherence tomography-based parameter measurement

Systems and method are provided for evaluating a biomechanical property of tissue. A shear wave generator is configured to induce a shear wave in the tissue. An optical coherence tomography (OCT) imager is configured to capture a B-scan frame of the tissue. An image processing component is configured to determine a frequency of the shear wave in the tissue from the B-scan frame of the tissue at each of a plurality of locations within the B-scan. A parameter calculation component is configured to calculate a value for the biomechanical property for a plural subset of the plurality of locations within the B-scan frame of the tissue from the determined frequency of the shear wave at each of the plural subset of the plurality of locations.

Measurement of biomechanical properties in an OCT image

Systems, methods, and other embodiments associated with gated optical coherence tomography (OCT) are described. One example method includes generating an image control signal to control an OCT apparatus to acquire an image of an embryonic heart at a specified point in time during a cardiac cycle of the embryonic heart. The method may also include controlling the OCT apparatus to acquire the image based on the image control signal. In different examples, the image may be acquired in vivo or from an excised heart that is paced. The OCT apparatus and the embryonic heart may be housed in an environmental chamber having a set of controllable environmental factors. Therefore, the method may include detecting and controlling the set of controllable environmental factors.

Growing community of inventors

Shaker Heights, OH, United States of America

Michael W Jenkins