Medimmune Limited

Albany Molecular Research, Inc.

Michael Van Brunt

Kenneth H Grabstein

Harold Meckler

Marcello Marelli

Paul F Vogt

Prasad Raje

Ben Littler

William Brady

Jeffrey C Johnson

Benjamin J Littler

Jeffrey Johnson

There is provided inter alia a process for stabilizing a eukaryotic cell line which expresses PylRS and tRNAPyl and which is suitable for incorporation of a gene encoding a target protein containing one or more non-natural amino acids encoded by a nonsense codon which comprises culturing said cell line under conditions in which the adverse effect of tRNAPyl expression on cell viability and/or cell growth is reduced or eliminated.

Cell lines

There are provided amino acid derivatives of formula V and VI as defined herein which are pyrrolysine analogs for use in bioconjugation processes.

Amino acid derivatives

There are provided pyrrolysine analogs of the formulae (X), (I), (II), (V), (VI), (VII) and (VIII), in which the a, b, d, X, Y, Z, FG, R, R, Rand Rare as defined in the claims, which are useful in bioconjugation processes and mutant proteins containing them.

Processes are disclosed for preparing piperidine derivative compounds of the formulae I, II or III: 

Process for production of carebastine

Processes are disclosed for preparing piperidine derivative compounds of the formulae I, II or III: The processes involve reacting a compound of formula Ia, IIa or IIIa with isobutyrate or an isobutyrate equivalent.

Growing community of inventors

Covington, WA, United States of America

Michael Van Brunt