Ionwerks, Inc.

University of California

The Texas A&m University System

Michael V Ugarov

J Albert Schultz

Thomas F Egan

Agnès Tempez

Valeri V Raznikov

Vladislav V Zelenov

Marina O Raznikova

David H Russell

Valerie E Vaughn

Yvon Le Beyec

John A McLean

Valeriy V Raznikov

Alexander R Pikhtelev

Gennadiy Savenkov

Kimberly A Prather

John Albert Schultz

Serge D Negra

Agnes Tempez

Serge Della-Negra

Valentin A Tarasenko

Hiroshi Furutani

Albert J Schultz

A new type of matrices for Matrix Assisted Laser Desorption and Ionization technique based on derivatized carbon clusters, which will be suitable for the analysis of peptides, proteins and other biomolecules by mass spectrometry which has several advantages over conventional matrices, such as optical sensitivity in a broad spectrum, low interference in the wide mass range and high analyte ionization efficiency.

Inorganic nanoparticle matrices for sample analysis

The content of the invention comprises a concept of reactor for isolated ion transformations induced by collisions with neutral species. This reactor is also an interface between mobility cell and orthogonal injection TOFMS based on supersonic adiabatic gas flow with variable controlled composition directed along the axis of a multipole ion guide with sectioned rods for possibility of creating of controlled distributions of RF, DC and AC rotating fields.

Neutral/ion reactor in adiabatic supersonic gas flow for ion mobility time-of-flight mass spectrometry

The content of the invention comprises a concept of multi-beam ion pre-selection from a single sample, coordinated mobility (against the gas flow) separation, cooling ions in supersonic gas flow and mass separation of thus low divergent ions by single or plural compact high-resolution orthogonal time-of-flight mass spectrometers both linear or reflectron type with controlled collision-induced dissociation (CID) and multi-channel data recording for the optimization of sample use in the analysis, and obtaining as much useful information about the sample as possible in a reasonably short time.

Multi-beam ion mobility time-of-flight mass spectrometry with multi-channel data recording

A method and apparatus for multiplexed data acquisition for gas-phase ion mobility coupled with mass spectrometry is described. Ion packets are injected into an ion mobility drift chamber at a rate faster than the ion mobility separation arrival time distribution. The convoluted arrival time distributions thus generated are deconvoluted by a mass spectrometer and post-processing algorithms.

Multiplex data acquisition modes for ion mobility-mass spectrometry

The present invention enhances the laser desorption of biological molecular ions from surfaces by creating a surface localized MALDI particle matrix by ion implantation of low energy ionized clusters (gold, aluminum, etc.) or chemically derivatized clusters into the near surface region of the sample. MALDI analysis of the intact biomolecules on the surface or within a narrow subsurface region defined by the implantation range of the ions can then be performed by laser desorption into a mass spectrometer or, in a preferred embodiment, into a combined ion mobility orthogonal time of flight mass spectrometer.

Gold implantation/deposition of biological samples for laser desorption two and three dimensional depth profiling of biological tissues

Neutral/Ion reactor in adiabatic supersonic gas flow for ion mobility time-of-flight mass spectrometry

The present invention relates generally to instrumentation and methodology for the characterization of chemical samples in solutions or on a surface which is based on modified ionization methods with or without adjustable pH and controllable H-D exchange in solution, in improved ion mobility spectrometer (IMS), a multi-beam ion per-selection of the initial flow, and coordinated mobility and mass ion separation and detection using a single or several independent time-of-flight mass spectrometers for different beams with methods for fragmenting ion mobility-separated ions and multi-channel data recording.

Multi-beam ion mobility time-of-flight mass spectrometer with bipolar ion extraction and zwitterion detection

Improved ion focusing for an ion mobility drift cell allows for improved throughput for subsequent detection such as mass detection. Improved focusing is realized by the use of alternating regions of high and low electric fields in the ion mobility drift cell.

Ion mobility TOF/MALDI/MS using drift cell alternating high and low electrical field regions

An ion mobility/mass spectrometry method and instrument using aerosolized samples and dual positive and negative mode detection is described. Sample preparation methods are also described.

MALDI-IM-ortho-TOF mass spectrometry with simultaneous positive and negative mode detection

Gold implantation/deposition of biological samples for laser desorption three dimensional depth profiling of tissues

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Houston, TX, United States of America

Michael V Ugarov