E. Heller & Company

Other

Abbott Diabetes Care Inc.

Therasense, Inc.

The University of Texas System

Sontra Medical Corporation

Massachusetts Institute of Technology

Michael V Pishko

Adam Heller

Ephraim Heller

Joseph Kost

Samir Suresh Mitragotri

Stephen C Rowe

Brian A Gregg

Gerard L Cote

Ioanis Katakis

Vamsi K Yadavalli

Wolfgang Kerner

Rebecca M Rounds

Hope T Beier

Matthew Davis

Bennett L Ibey

Robert Samuel Langer

R Rox Anderson

Ryan Thomas Russell

Daniel Blankschtein

Mark E Johnson

Kaushik Sirkar

Gerard L Cot&eacute;

A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow 'one-point' in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably three or four-layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentration-to-current transducing layer is overcoated with an electrically insulating and glucose flux limiting layer (second layer) on which, optionally, an immobilized interference-eliminating horseradish peroxidase based film is deposited (third layer). An outer (fourth) layer is biocompatible.

Subcutaneous glucose electrode

A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow 'one-point' in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentration-to-current transducing layer can be overcoated with an electrically insulating and glucose flux limiting layer (second layer) on which, optionally, an immobilized interference-eliminating horseradish peroxidase based film is deposited. An outer layer is preferably biocompatible.

This disclosure relates to a method of measuring a glucose concentration metric or a glucose metric in a patient by contacting an implantable glucose-sensing device with a test sample, which may be in the patient, under conditions that permit a sugar-binding molecule and a functionalized polymer or nano-particle ligand present throughout the matrix of a hydrogel to interact in a glucose-dependent manner to produce an optical signal resulting from quenching of a first fluorophore linked to the ligand or sugar-binding molecule and having a fluorescent emission spectrum quenched upon binding or release of glucose. Next the first fluorophore may be excited with light of a certain wavelength. Then at least one wavelength of light in the glucose-dependent optical signal from the fluorophore may be detected with a detector to produce a detected light signal, which may be processed to produce a glucose metric, such as a glucose concentration metric.

Method for glucose monitoring using fluorescence quenching

This disclosure relates to systems, devices, and methods of sensing an analyte. An implantable sensor may be contacted with a test sample under conditions that permit a binding protein and a ligand of the sensor to interact in an analyte-dependent manner to produce an analyte-dependent signal, and (b) detecting the analyte-dependent signal with a detector. A binding protein may reversibly bind an analyte and/or a ligand. A binding protein may have a higher binding affinity for an analyte than for a ligand. A binding protein and a ligand may each include a fluorophore, the absorption and/or emission properties of which may change in an analyte-dependent manner. A binding protein and/or a ligand may be bound to an active or inactive substrate. Some embodiments of systems, devices, and methods may be practiced in vitro, in situ, and/or in vivo. Systems and/or devices of the disclosure may be configured to be wearable.

Implantable system for glucose monitoring using fluorescence quenching

A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow &ldquo;one-point&rdquo; in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably three or four-layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentration-to-current transducing layer is overcoated with an electrically insulating and glucose flux limiting layer (second layer) on which, optionally, an immobilized interference-eliminating horseradish peroxidase based film is deposited (third layer). An outer (fourth) layer is biocompatible.

Methods and devices for application of ultrasound to a small area of skin for enhancing transdermal transport. An ultrasound beam having a first focal diameter is channelled into a beam having a second, smaller diameter without substantial loss of energy. Higher energy ultrasound can be used while causing less pain. Alternatively, ultrasound energy is applied through a vibrating element positioned just contacting, above or extending into the skin. Use of the element facilitates extraction of analyte and may enhance drug delivery. A two step noninvasive method involves application of ultrasound to increase skin permeability and removal of ultrasound followed by transdermal transport that can be further enhanced using a physical enhancer.

Ultrasound enhancement of transdermal transport

Provided are a variety of chemically sensitive, stable (insoluble over a specified period of time), nontoxic, and non-antigenic hydrogel particles which undergo a measurable change in at least one electrochemical or optical property as a function of interaction with one or more substance(s) to be detected. Also provided are methods of using these hydrogel particles to detect one or more selected analytes, and in certain aspects detect one or more analytes in vivo. Further provided are devices used to detect and measure the optical or electrochemical changes.

Compositions and methods for analyte detection

A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucoses is described. The electrode is designed to allow &ldquo;one-point&rdquo; in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably three or four-layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentration-to-current transducing layer is overcoated with an electrically insulating and glucose flux limiting layer (second layer) on which, optionally, an immobilized interference-eliminating horseradish peroxidase based film is deposited (third layer). An outer (fourth) layer is biocompatible.

Electrochemical analyte measurement system

Growing community of inventors

Austin, TX, United States of America

Michael V Pishko