Schering Aktiengesellschaft

Michael Topert

Henry Laurent

Helmut Hofmeister

Klaus Annen

Rudolf Wiechert

Petra Rach

Walter Elger

Dieter Bittler

Gerald Kirsch

Thomas Brumby

Ruth Thieroff-Ekerdt

Peter Esperling

Jurgen Spona

Joachim Daum

Gerhard Siewert

Hartmut Seliger

Christopher G Earnshaw

Hans J Zentel

Glucocorticoids

Hydroxyalkane carboxylic acid derivatives of general formula I ##STR1## in which n means the numbers 7 to 18,

Hydroxyalkane carboxylic acid derivatives and their production

Androstane derivatives of the general Formula I ##STR1## in which R.sub.1 represents a hydrogen atom or a methyl group,

Androstane derivatives

6.alpha.,16.beta.-Dimethyl corticoids of general Formula I ##STR1## wherein the bond .sub.-------- is a single bond or a double bond, and

6.alpha., 16.beta.-dimethyl corticoids and their anti-inflammatory use

1.alpha.,2.alpha.-methylene-6-methylene- and 6.alpha.-methylpregnenes of Formula I ##STR1## wherein A is ##STR2## and R is a hydrogen atom, an acyl group, or an alkoxymethyl group are strong antiandrogens, and suitable for the topical treatment of seborrhea, acne, alopecia, and hirsutism.

1.alpha.,2.alpha.-methylene-6-methylene- and 6.alpha.-methylpregnenes,

6.alpha.,16.alpha.-Dimethyl corticoids of general Formula I ##STR1## wherein the bond  is a single bond or a double bond,

6.alpha., 16.alpha.-dimethyl corticoids

A process for cleavage of a protein (a) sequence from a protein containing the tetrapeptide sequence Pro-Xyz-Gly-Pro, wherein Xyz can be any desired amino acid, and reacting the protein with a collagenase whereby the Xyz-Gly bond is selectively cleaved, thereby producing a protein with the sequence Gly-Pro at its N-terminal end; or (b) containing the sequence Met-Pro; and subsequently selectively cleaving the glycine or methionine residue from the N-terminal end of the product protein with an aminoacylproline aminopeptidase and selectively cleaving the proline residue therefrom with a proline aminopeptidase; or subsequently selectively cleaving the Gly-Pro or Met-Pro residue from the N-terminal end of the produced protein using a postproline dipeptidylaminopeptidase. In a special embodiment, the protein which is split contains the sequence -Zxy-Uvw-Pro- on the carboxy end of the sequence Pro-Xyz-Gly-Pro, wherein Zxy and Uvw are independently any natural amino acid in the genetic code except Pro, whereby after the collagenase reaction, there is produced a protein with the sequence Gly-Pro-Zxy-Uvw-Pro at the N-terminal end. This is split by subsequently selectively cleaving the Pro-Zxy-bond by treating the protein with postproline dipeptidylaminopeptidase (PPDA), thereby producing protein having the sequence Zxy-Uvw-Pro at its N-terminal end, followed by cleaving the Zxy acid from the resultant protein by treating the latter with leucine aminopeptidase.

Process for the specific cleavage of protein sequences from proteins

Stable preparations of sensitized erythrocytes are provided by simultaneously admixing washed erythrocytes in a buffered aqueous solution of glutaraldehyde, antigen or antigen-protein conjugate and formaldehyde. After washing, the separated sensitized erythrocytes can be lyophilized and thereby become stable at room temperature.

Growing community of inventors

Berlin, Germany

Michael Topert