454 Life Sciences Corporation

'454 Corporation

Michael T Ronan

Jonathan Marc Rothberg

John Harris Leamon

Michael Weiner

Yi-Ju Chen

Kenton L Lohman

Maithreyan Srinivasan

Kenton Lohman

John H Leamon

This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand of nucleic acid; annealing a second blocked primer to a second strand of nucleic acid; elongating the nucleic acid along the first strand with a polymerase; terminating the first sequencing primer; deblocking the second primer; and elongating the nucleic acid along the second strand.

Double ended sequencing

Growing community of inventors

New Haven, CT, United States of America

Michael T Ronan