Monsanto Technology LLC

Dekalb Genetics Corporation

Michael T Mann

Brenda A Lowe

David D Songstad

Brandon Davis

William L Petersen

Jyoti R Rout

David R Duncan

Anisha Akula

Wanggen Zhang

Joel B Wilks

Michael Spencer

Lubomyr Kucher

Paul C Anderson

Thomas R Adams

Albert P Kausch

Whitney R Adams, Jr

William J Gordon-Kamm

Peter M Orr

Richard J Daines

Yuechun Wan

T Michael Spencer

Nancy G Willetts

Tishu Cai

David C Warner

Whitney Adams

Anita Otal

Richard A Yingling

Shubha Subbarao

James R Larkins

David A Walters

Paul S Chomet

Peggy G Lemaux

James V O'Brien

Catherine J Mackey

Lucille B Laccetti

Sheryl A Chambers

Thomas B Rice

William G Start

Michael A Stephens

Mary L Mangano

Christopher E Flick

Emil M Orozco

Donald S Walters

Hongyi Zhang

Susan J Zachwieja

Clayton S Vetsch

Kimberly Glassman

A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.

Methods for producing transgenic plants

The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.

Method and apparatus for substantially isolating plant tissues

Production of transformed plants is achieved by placing-inoculated explants directly onto a medium containing selective agents for suppressing growth ofand killing non-transformed explant cells.

The present invention relates to a novel transformation system for generating transformed corn plants. In particular, the invention relates to a rapid selection system at an elevated temperature that allows faster and more efficient transformation.

Methods for corn transformation

This invention describes a simple method useful for the excision and isolation of maize immature embryos. The embryos are useful for plant tissue culture and transformation methods.

Method for excision of plant embryos for transformation

This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs thebar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof

Growing community of inventors

Groton, CT, United States of America

Michael T Mann