Harvard College

Merck Patent Gmbh

Cancer Research Technology Limited

Michael Super

Donald Elliot Ingber

Alexander L Watters

Mark J Cartwright

Martin M Rottman

Daniel Levner

George M Church

Jeffrey Charles Way

Jehyuk Lee

Brendon Dusel

Jonathan Harry Davis

Julia B Berthet

Julie A Tomolonis

Philip T Snell

Dinah R Super

Joanna Aizenberg

Tak Sing Wong

Je-Hyuk Lee

Benjamin Hatton

David J Mooney

Edward J Doherty

Philseok Kim

Karen A Sinclair

Evangelia Murray

Nazita Gamini

Omar Abdel-Rahman Ali

Pascal Andre Stein

Alexander G Stafford

Daniel C Leslie

Pascal André Stein

Anna Waterhouse

Benjamin Seiler

Amanda Graveline

Des White

Joo Hun Kang

Thomas Doyle

Olivier Y F Henry

Melissa Rodas

Joo-Hun Kang

Harry Scott Driscoll

John Samuel Workman

Martin Rottman

Joanna Alzenberg

Michael Alzenberg

Evangelina Murray

Donald Elliott Ingber

Jonathan H Davis

Julia Bellows Berthet

Tohid Didar

Methods of analyzing nucleic acids of a cell are provided.

Compositions and methods for analyte detection

Described herein are heme-binding compositions and methods relating to their use, for example methods of treatment of sepsis and rhabdomyolysis.

Engineered heme-binding compositions and uses thereof

This disclosure provides, e.g., methods for coupling Formation of Surface Carboxylates on PES an entity to a solid substrate. The method can comprise treating the with Exposure Time substrate with a plasma, e.g., a CO2 plasma, to increase its reactivity. The entity can be, e.g., a biological polymer that binds a microbe. Substrates produced by these methods can be used in a variety of applications, including hemodialysis and diagnostic assays.

Aqueous biomolecule coupling on CO2-plasma-activated surfaces

Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can not only bind or capture of a microbe or microbial matter thereof, but they also have improved capability (e g, enhanced sensitivity or signal intensity) of detecting a microbe or microbial matter. Thus, the microbe-targeting molecules, microbe-targeting articles, and/or the kit described herein can be used in various applications, e.g., but not limited to assays for detection of a microbe or microbial matter, diagnostic and/or therapeutic agents for diagnosis and/or treatment of an infection caused by microbes in a subject or any environmental surface, and/or devices for removal of a microbe or microbial matter from a fluid.

Microbe-binding molecules and uses thereof

Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

Engineered microbe-targeting molecules and uses thereof

Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can bind or capture of a microbe or microbial matter thereof, and can thus be used in various applications, such as diagnosis or treatment of an infection caused by microbes in a subject or any environmental surface.

CRP capture/detection of gram positive bacteria

The present invention provides vaccine compositions and methods of producing such compositions. Other embodiments of the invention include methods of treating a pathogen infection, methods of vaccinating a subject against a pathogen infection, and methods for treating an antibiotic-resistance bacterial infection in a subject in need thereof. In further embodiments, the invention includes methods of decreasing the level of a pathogen in a subject having a pathogen infection, methods of increasing the surviving rate of a subject having a pathogen infection, methods of reducing the level of pain associated with a pathogen infection, and methods of reducing the level of distress associated with a pathogen infection in a subject in need thereof. Novel scaffold compositions and opsonin-bound or lectin-bound pathogen compositions, and uses thereof, are also provided herein.

Pathogen vaccines and methods of producing and using the same

Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

The inventions provided herein relate to detection reagents, compositions, methods, and kits comprising the detection reagents for use in detection, identification, and/or quantification of analytes in a sample. Such detection reagents and methods described herein allow multiplexing of many more labeled species in the same procedure than conventional methods, in which multiplexing is limited by the number of available and practically usable colors.

Growing community of inventors

Lexington, MA, United States of America

Michael Super