Applera Corporation

Bodenseewerk Perkin-elmer Gmbh

Universitaet Tuebingen

Michael Steinwand

Timothy G Geiser

Henry Klemm

J&uuml;rgen Wulf

H Michael Wenz

Alexander Jung

Gunter Gauglitz

Simone Guenther

Ivo Stemmler

Andreas Brecht

Apparatus and method for performing sequential bioassays with microfluidic transfers.

Multi-step bioassays on modular microfluidic application platforms

The invention concerns a method for quantitative or qualitative determination of an analyte or its interaction or reaction kinetics in a system with at least two different phases, comprising the step of taking at least one measurement signal from at least one of the phases, whereby the different phases are present in parallel when taking the signal and whereby each measurement signal is attributed to one of at least two phases. In addition, the invention concerns a sample carrier, in particular for use in the method constituting the invention with one or more wells. The sample carrier is characterized by the fact that at least a portion of the sample carrier at least in the range of one or more wells is coated with fluorescence-quenching material.

Quantitative determination of analytes in a heterogeneous system

A light scanning device for exciting and detecting secondary light, especially fluorescent light, on a sample, comprising a light emission device for emitting exciting light with a wavelength suitable for exciting secondary light on or in said sample, a focussing optics for focussing the exciting light on a subarea of said sample, a sample holding device for releasably holding the sample, a detection unit comprising a detection optics for the secondary light emitted by the sample in response to excitation and a detector device for converting the detected and imaged secondary light into electric signals. In the case of conventional known light scanning devices, scanning is carried out by means of a deflection unit consisting of tilting mirrors. Due to the long path of the light beam, positioning inaccuracies of the tilting mirrors result in major position inaccuracies of the scanning ray bundle on the surface of the sample. For avoiding this disadvantage of the prior art, the light scanning device according to the present invention makes use of a sample holding device which is adapted to be rotated for rotating the sample relative to the exciting light in such a way that different subareas of said sample can be excited by means of the exciting light so as to emit secondary light. Due to the mechanical rotary movement of the sample, a deflection of the scanning light beam relative to the optical axis is not necessary so that precise positioning of the scanning ray bundle on the sample is possible.

Light-scanning device

Growing community of inventors

Owingen, Germany

Michael Steinwand