Leland Stanford Junior University

Zymergen Inc.

Ajinomoto Co., Ltd.

Michael Shareef Siddiqui

Christina D Smolke

Steven M Edgar

Alexander Glennon Shearer

Cara Ann Tracewell

Benjamin Mijts

Christine Roche

Stefan De Kok

Sheetal Modi

Murtaza Shabbir Hussain

Nicolaus Herman

Franklin Lu

Host cells that are engineered to produce benzylisoquinoline alkaloid (BIAS) precursors, such as norcoclaurine (NC) and norlaudanosoline (NL), are provided. The host cells may have one or more engineered modifications selected from: a feedback inhibition alleviating mutation in a enzyme gene; a transcriptional modulation modification of a biosynthetic enzyme gene; an inactivating mutation in an enzyme; and a heterologous coding sequence. Also provided are methods of producing a BIA of interest or a precursor thereof using the host cells and compositions, e.g., kits, systems etc., that find use in methods of the invention.

Benzylisoquinoline alkaloid (BIA) precursor producing microbes, and methods of making and using the same

The present disclosure describes the engineering of microbial cells for fermentative production of histamine and provides novel engineered microbial cells and cultures, as well as related histamine production methods.

Engineered biosynthetic pathways for production of histamine by fermentation

Host cells that are engineered to produce benzylisoquinoline alkaloid (BIAs) precursors, such as norcoclaurine (NC) and norlaudanosoline (NL), are provided. The host cells may have one or more engineered modifications selected from: a feedback inhibition alleviating mutation in a enzyme gene; a transcriptional modulation modification of a biosynthetic enzyme gene; an inactivating mutation in an enzyme; and a heterologous coding sequence. Also provided are methods of producing a BIA of interest or a precursor thereof using the host cells and compositions, e.g., kits, systems etc., that find use in methods of the invention.

The present disclosure describes the engineering of microbial cells for fermentative production of tyramine and provides novel engineered microbial cells and cultures, as well as related tyramine production methods.

Engineered biosynthetic pathways for production of tyramine by fermentation

A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified to have a Bacteroidetes O-methyltransferase (OMT) gene, especially an OMT gene encoding OMT having a specific mutation.

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San Mateo, CA, United States of America

Michael Shareef Siddiqui