Pfizer Corporation

Boston Medical Center Corporation

Repligen Corporation

Michael Shamashkin

Nathan Higginson-Scott

Zong Sean Juo

Stefano V Gulla

Stephen Berasi

David J Salant

Sreekumar R Kodangattil

Janet Elizabeth Buhlmann

Xueping Fan

Weining Lu

Matthew Russo

Keith A Johnson

Alexander H Slocum

Jonathan Coffman

Ranganathan Godavarti

Jason C Rouse

Travis R Ward

Mary Beth Switzer

Daniel P Witt

Penelope Jane Sharpe

Stacey B Weston

Michael Anthony Jankowski

Adrian Lowe

Wendy Carol Piacenza

William J Wilde

Jonathan Lee Coffman

The invention provides recombinant Roundabout Receptor 2 (ROBO2) proteins designed to bind SLIT ligands and prevent their binding to ROBO2 cell surface receptors. Also provided are methods for use of these recombinant ROBO2 proteins.

Recombinant ROBO2 proteins, compositions, methods and uses thereof

Compositions are provided comprising recombinant variants of the human clotting Factor Xa. Such compositions include a wide variety of isoforms and post-translational modifications of FXa and are useful for treating subjects in need of hemostasis.

Methods for purifying FXa variant proteins

A method of making and loading a chromatography column includes selecting a column tube having an appropriate elasticity, and inner diameter and length, selecting appropriately sized flow distributors, a second flow distributor having a diameter that is larger than the inner diameter of the tube, permanently securing a first flow distributor to a first end of the tube, loading packing medium into the tube, inserting the second flow distributor into a second end of the tube to drive the second flow distributor into the tube to form a sealed chamber within the tube, adjusting the longitudinal position of the second flow distributor within the tube by applying a force to the second flow distributor until it reaches a desired location within the tube, and/or forcing liquid into the chamber to move the second flow distributor and permanently securing the properly positioned second flow distributor within the tube.

Chromatography columns

The present disclosure provides methods for purifying products from a fluid. In some embodiments, provided purification methods use a combination of purification modes (e.g., protein A and ion exchange) operated in tandem, wherein at least one of the modes utilizes weak partitioning. In some embodiments, provided purification methods operate under robust conditions in which a degree of binding between a product and resin is maintained despite variations in operating parameters.

Tandem purification of proteins

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Woburn, MA, United States of America

Michael Shamashkin