University of Pittsburgh

Michael S Sacks

Christopher C Capelli

Michael B Chancellor

Johnny Huard

Steve Chung

Mohammed S El-Kurdi

Sharan D Ramaswamy

Steven M Boronyak

George C Engelmayr, Jr

David E Schmidt

Michael B, Chancellor

A rapid method for preparing stem cell and physiologically acceptable matrix compositions for use in tissue and organ repair is described. Compared with previous tissue engineering materials, the stem cell-matrix compositions of the present invention do not require long-term incubation or cultivation in vitro prior to use in in vivo applications. The stem cells can be from numerous sources and may be homogeneous, heterogeneous, autologous, and/or allogeneic in the matrix material. The stem cell-matrix compositions provide point of service utility for the practitioner, wherein the stem cells and matrix can be combined not long before use, thereby alleviating costly and lengthy manufacturing procedures. In addition, the stem cells offer unique structural properties to the matrix composition which improves outcome and healing after use. Use of stem cells obtained from muscle affords contractility to the matrix composition.

Rapid preparation of stem cell matrices for use in tissue and organ treatment and repair

Described herein is a bioreactor system and modules capable of developing physiologically relevant fluid-induced shear stresses and regionally specific flow patterns to scaffold specimens and which can couple these stresses to cyclic flexure and/or stretch states. Methods of use of the bioreactor system and module also are provided.

Flow-stretch-flexure bioreactor

The invention is directed to preparation of stem cell and physiologically acceptable matrix compositions. Compared with previous tissue engineering materials, the stem cell-matrix compositions of the present invention do not require long-term incubation or cultivation in vitro prior to use in vivo applications. The stem cells can be from numerous sources and may be homogeneous, heterogeneous, autologous, and/or allogeneic in the matrix material. The stem cell-matrix compositions provide point of service utility for the practitioner, wherein the stem cells and matrix can be combined not long before use, thereby alleviating costly and lengthy manufacturing procedures. In addition, the stem cells offer unique structural properties to the matrix composition which improves outcome and healing after use. Use of stem cells obtained from muscle affords contractility to the matrix composition.

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Pittsburgh, PA, United States of America

Michael S Sacks