Vib Vzw

Oxford Nanopore Technologies Limited

Vrije Universiteit Brussel

The Ohio State University

Michael Robert Jordan

Lakmal Nishantha Jayasinghe

Elizabeth Jayne Wallace

Richard George Hambley

Han Karel Remaut

Pratik Raj Singh

John Joseph Kilgour

Sander Egbert Van Der Verren

Nani Van Gerven

Peixuan Guo

Zhouxiang Ji

Han Remaut

The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.

Protein pores

Provided is a method of characterising a polynucleotide using a transmembrane pore, wherein the pore is a double pore comprising a first Csg G pore, or a homologue thereof, and a second CsgG pore, or a homologue thereof.

Transmembrane pore consisting of two CsgG pores

Disclosed herein are compositions and methods that involve inserting connector protein channels of bacteriophage DNA packaging motors into copolymeric membranes via liposome-polymer fusion, which can be used as nanopore sensors for biomedical applications such as high throughput protein sequencing or cancer diagnosis. For example, disclosed are compositions comprising a copolymeric membrane into which a connector protein channel of a bacteriophage packaging motor has been inserted.

Bacteriophage-derived nanopore sensors

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Oxford, United Kingdom

Michael Robert Jordan