Integenx, Inc.

Pall Corporation

Michael Recknor

Stevan Bogdan Jovanovich

William D Nielsen

Ezra Van Gelder

Omar EL-Sissi

David S Cohen

David Eberhart

Mattias Vangbo

Richard Joseph Belcinski

Gregory Bogdan

Lars Majlof

David W Wyrick

Robert F Zuk

Hong Tan

Krista Leah Witte

Scott Lockard

Sae Choo

David Wyrick

This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

Sample preparation, processing and analysis systems

The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.

Universal sample preparation system and use in an integrated analysis system

An apparatus and method for packaging of an optical sensing fiber is disclosed. The apparatus includes a substrate with a plurality of openings, and each opening is configured for holding an optical sensing assembly. The assembly is positioned in the opening with a tip of the assembly extending through the opening to be suspended from the substrate. In addition, openings are arranged so the assembly positioned therein avoids contacting another assembly positioned therein. The apparatus can include a support member for supporting the substrate and positioning the substrate so the tip of the assembly suspended from the opening in the substrate contacts solution in one of a plurality of wells in a container adjacent to the substrate. The assembly can be configured for preparing of the optical assembly for assay. An agitation assembly for agitating the container to create flow of the solution in the container wells over an optical sensing assembly is also disclosed.

Tip tray assembly for optical sensors

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Oakland, CA, United States of America

Michael Recknor