Mycogen Corporation

Michigan State University

Hycogen Corporation

Michael R Pollard

Kenneth Edwin Narva

H Ernest Schnepf

George E Schwab

Guy A Cardineau

Mark E Knuth

Tracy Ellis Michaels

Stacey Finstad Lee

Joanna Dojillo

John B Ohlrogge

Xiaoming Bao

Rod Aaron Herman

Lisa M Stamp

Anne Milcamps

David A Pan

Paula Diehl

Paula Burmeister

Timothy Durrett

The present invention relates to novel diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes encoding proteins having diacylglycerol acetyltransferase activity, specifically for transferring an acetyl group to a diacylglycerol substrate to form acetyl-Triacylglycerols (ac-TAGS), for example, a 3-acetyl-1,2-diacyl-sn-glycerol. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms. The present invention also relates to methods of using novel diacylglycerol acyltransferase genes and proteins, including their expression in transgenic organisms at commercially viable levels, for increasing production of 3-acetyl-1,2-diacyl-sn-glycerols in plant oils and altering the composition of oils produced by microorganisms, such as yeast, by increasing ac-TAG production. Additionally, oils produced by methods of the present inventions comprising genes and proteins are contemplated for use as biodiesel fuel, in polymer production and as naturally produced food oils with reduced calories.

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Cyclopropane fatty acid synthase genes and polypeptides are described. Plants are transformed with such genes to produce such polypeptides.

Plant cyclopropane fatty acid synthase genes, proteins, and uses thereof

The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences that encode these proteins. In preferred embodiments, these pesticidal proteins have molecular weights of approximately 40-50 kDa and of approximately 10-15 kDa.

Pesticidal proteins

The present invention provides cyclopropane fatty acid synthase genes and proteins, and methods of their use. The present invention encompasses both native and recombinant wild-type forms of the synthase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type synthase. The present invention also provides methods of using cyclopropane fatty acid synthase genes and proteins, including in their expression in transgenic organisms and in the production of cyclopropane fatty acids in plant oils, and in particular seed oils.

The present invention relates to diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes and proteins that exhibit both long-chain acyltransferase and acetyltransferase activity. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type transferase. The present invention also relates to methods of using diacylglycerol acyltransferase genes and proteins, including in their expression in transgenic organisms and in the production of acetyl-glycerides in plant oils, and in particular seed oils.

Diacylglycerol acyltransferase genes, proteins, and uses thereof

The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences which encode these proteins. More specifically, in preferred embodiments, pesticidal proteins of approximately 40-50 kDa and of approximately 10-15 kDa are used for controlling corn rootworms. Also described are novel pesticidal isolates of

The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences that encode these proteins. In preferred embodiments, these pesticidal protein have molecular weights of approximately 40&#x2013;50 kDa and of approximately 10&#x2013;15 kDa.

The subject invention concerns new classes of insecticidal proteins obtainable from, and polynucleotides that encode these proteins. The subject invention also includes transgenic cells and plants that produce these proteins. The proteins are preferably in the 10-15 kDa and 40-50 kDa size range.

Pesticidal toxins

The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encoded these toxins. Also described are novel pesticidal isolates of

The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encode these toxins. Also described are novel pesticidal isolates of

The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encode these toxins. Also described are novel pesticidal isolates of Bacillus thuringiensis.

Growing community of inventors

Okemos, MI, United States of America

Michael R Pollard