Encodia, Inc.

Stratagene

Michael Phillip Weiner

Mark S Chee

Kevin L Gunderson

Lei Shi

Norihito Muranaka

John M Beierle

Robert C James

Luca Monfregola

Joseph A Sorge

Dan Shoemaker

A method for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding is disclosed.

Macromolecule analysis employing nucleic acid encoding

Kits and methods of using the kits for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding are disclosed. The sample analysis kits employ nucleic acid encoding and/or nucleic acid recording of a molecular interaction and/or reaction, such as recognition events (e.g., between an antigen and an antibody, between a modified terminal amino acid residue, or between a small molecule or peptide therapeutic and a target, etc.). Additional barcoding reagents, such as those for cycle-specific barcoding (e.g., 'clocking'), compartment barcoding, combinatorial barcoding, spatial barcoding, or any combination thereof, may be included in the kits. The sample may comprise macromolecules, including peptides, polypeptides, and proteins, and the recording may generate molecular interaction and/or reaction information, and/or polypeptide sequence information. The kits may be used in high-throughput, multiplexed, and/or automated analysis, and are suitable for analysis of a proteome or subset thereof.

Kits for analysis using nucleic acid encoding and/or label

Methods and Kits for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding are disclosed. The sample analysis kits employ nucleic acid encoding and/or nucleic acid recording of a molecular interaction and/or reaction, such as recognition events (e.g., between an antigen and an antibody, between a modified terminal amino acid residue, or between a small molecule or peptide therapeutic and a target, etc.). Assays that do not require the cyclic transfer of information between a coding tag and a recording tag are also disclosed, including single cycle assays.

Methods and kits using nucleic acid encoding and/or label

A composition comprising a family of oligonucleotides all of the same length, said family defined by a nucleotide sequence formula containing six to eight nucleotide positions, one to three of said positions of the formula identifying positions of nucleotide variation among the family members, the remaining nucleotide positions each identifying a nucleotide that is the same in all members of the family.

Oligonucleotide families useful for producing primers

A method for directionally cloning an insert DNA fragment into a target sequence using differential phosphorylation is disclosed, Monophosphorylated PCR fragments are directionally cloned into a monophosphorylated plasmid, Methods for directionally cloning non-PCR fragments into target DNA sequences are also discussed.

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San Diego, CA, United States of America

Michael Phillip Weiner