American Cyanamid Company

The Picower Institute for Medical Research

Michael Peter Vitek

Jack Steven Jacobsen

Anthony Cerami

Peter C Ulrich

Richard J Bucala

Helen Vlassara

Xini Zhang

Compositions and methods for stimulating amyloid removal in

This application describes a purified and isolated fragment of a nucleic acid molecule encoding an amyloid precursor mutein, wherein the fragment comprises a nucleic acid sequence encoding at least one marker and a nucleic acid sequence of about 419, about 475 or about 494 amino acid residues in which a portion thereof encodes a .beta.-amyloid protein domain. Also described is a method for screening for a compound which reduces the formation of .beta.-amyloid protein.

Amyloid precursor proteins and method of using same to assess agents

This disclosure concerns a method of detecting the presence of a polypeptide in a sample wherein the polypeptide is encoded by a fragment of a nucleic acid molecule encoding an amyloid precursor mutein and the fragment comprises a sequence encoding at least one marker and a sequence encoding about 419 amino acid residues of the APP-695 isoform, about 475 amino acid residues of the APP-751 isoform or about 494 amino acid residues of the APP-770 isoform. The method encompasses contacting the sample with an antibody, which specifically binds the marker or the amyloid precursor mutein, under suitable conditions to favor the formation of an antibody-antigen complex and detecting the presence of any complex thus formed. The disclosure also deals with the method employing the above nucleic acid fragment wherein the amino acid residues from position 11 to position 28 are deleted from the portion of the sequence encoding the .beta.-amyloid protein domain and in which an alanine substitution at a phosphorylation site within the cytoplasmic domain of the amyloid precursor protein is encoded.

Method of detecting amyloid precursor proteins

Growing community of inventors

East Norwich, NY, United States of America

Michael Peter Vitek