Life & Brain Gmbh

Rheinische Friedrich-Wilhelms-Universität

Rheinische Friedrich-wilhelms-universitaet

Michael Peitz

Oliver Brüstle

Harald Neumann

Kristin Roy

Frank Edenhofer

Manal Hadenfeld

Marc-Christian Thier

A method for obtaining human microglial precursor cells, comprising: (a) providing a cell population comprising neural precursor cells, wherein the cell population is obtainable from embryoid bodies differentiated from human pluripotent stem cells; (b) differentiating the cell population comprising neural precursor cells into microglial precursor cells by culturing in medium comprising a growth factor selected from the group consisting of insulin and insulin-like growth factors; (c) expanding and enriching microglial precursor cells in medium comprising a growth factor selected from the group consisting of insulin and insulin-like growth factors and 10 to 150 ng/ml GM-CSF; and (d) isolating microglial precursor cells comprising CD45-positive cells.

Method for obtaining human microglial precursor cells from pluripotent stem cells

The invention relates to a fusion protein and a method for the generation of the fusion protein of the invention. Further, the invention relates to the use of the fusion protein of the invention for the generation of induced pluripotent cells. Moreover, the invention relates to a composition comprising at least one fusion protein of the invention.

Fusion protein and use thereof

Growing community of inventors

Bonn, Germany

Michael Peitz