Vertex Pharmaceuticals, Inc.

Michael Paul Emery

James Edward Sinclair

Sanjay Shivayogi Magavi

Minh Vuong

Brian Grot

Jack Barger

Matthew Charles Wallace

Raymond J Gonzales

Alec Tate Harootunian

Philip Emanuel Salzmann

Javier Humberto Flores

Todd N Bennett

Jorge Arturo Ayala

Disclosed herein are systems and methods for the developing of immuno-stained biological samples. The systems disclosed herein are automated and are configured to control one or more steps of the developing procedure. Reagents may be added using automatic syringe dispensing. Reagent temperature, reagent stirring, and wash procedures are programmable and can be separately controlled for separate immuno-staining procedures that are performed at the same time.

Automated developer for immuno-stained biological samples

Apparatuses and methods for handling a portion of a tissue sample when sectioned by a microtome. The apparatuses include a container, a tissue sample holder in the container, and one or more outlets configured to allow flow of a fluid from the container. The flow through the outlet causes a portion of the tissue sample sectioned by a microtome to move into the outlet. The methods include sectioning one or more portions of a tissue sample, and flowing a fluid past the tissue sample to cause the one or more portions of the tissue sample to move away from the tissue sample and toward at least one fluid outlet.

Device to collect and segregate tissue samples sectioned on a microtome

Methods and devices for controlled evaporation of solvent from a solution are provided. In one embodiment, a method for controlled evaporation of solvent from at least one solution includes filling a selected first set of wells in a multi-well plate with a first solution comprising at least one first solvent. The method also includes attaching an inlet manifold to the plate, the inlet manifold comprising an inlet port connected to and in fluid communication only with each of the wells in the selected first set of wells. The method further includes attaching an exhaust manifold to the plate, the exhaust manifold comprising an exhaust port connected to and in fluid communication only with each of the wells in the selected first set of wells. The method also includes introducing a gas into the inlet port and removing solvent vapor from the selected first set of wells.

Methods for crystallization by controlled evaporation

Methods and devices for controlled evaporation of solvent from a solution are provided. In one embodiment, the device can control evaporation of different solutions comprising solvents of dissimilar volatilities simultaneously. In another embodiment, control over the flow of solvent vapor out of the device is provided by selecting the pressure at an inlet port and an exhaust port of the device. In yet another embodiment, control over the flow of solvent vapor out of the device is provided by selecting the size of a disk flow control orifice positioned between the wells and a vapor removal outlet of the device. In still another embodiment, control over the flow of solvent vapor out of the device is provided by the opening and closing of the vapor removal outlet of the device. In yet a further embodiment, the rate of evaporation of solvent is controlled by controlling the temperature of the solution. The flow rate control mechanisms can be used alone or in any combination to control evaporation of one or more solvents from solutions.

Devices for crystallization by controlled evaporation

An air displacement pipetter comprises a guidance sleeve positioned within a hole in a cylinder block and a piston within a portion of the guidance sleeve a piston penetrating the cylinder block, a spring energized seal disposed within the cylinder block, a seal capture plate contacting the spring energized seal, a carrier plate, a pipette tip attached to the carrier plate, and a manifold plate between said carrier plate and said cylinder block.

Air displacement pipetter

A liquid level sensor comprises a plurality of electrodes arranged in a defined relationship with respect to a container filling trajectory, a signal generator coupled to one or more of the plurality of electrodes, a signal receiver coupled to one or more of the plurality of electrodes, and a sensing circuit coupled to at least one of the signal generator and signal receiver. The sensing circuit is configured to produce an output indicative of electrical resistance between at least one pair of the plurality of electrodes. The sensing circuit may be associated with the signal receiver and/or with the signal generator.

Growing community of inventors

Santee, CA, United States of America

Michael Paul Emery