University of California

The J. David Gladstone Institutes

Michael P Sherman

Warner C Greene

Peter Henklein

Ulrich Schubert

Jay M Milstein

Ronaldo B Hipolito

Victor Wray

Uwe Tessmer

Carlos Mc De Noronha

Karsten Bruns

The present invention provides screening methods for identifying a compound that induces loss of the lentiviral protein Vpr; screening methods for identifying compounds that inhibit the peptidyl-prolyl cis/trans isomerase (PPIase) activity of a protein that catalyzes cis-trans isomerization of cis-peptidylprolyl bonds in Vpr; and compounds identified by the screening methods. The compounds are useful for treating a lentiviral infection. The present invention further provides methods of inducing loss of the lentiviral protein Vpr; methods of inhibiting lentivirus viral replication; and methods of treating a lentivirus infection in an individual. The methods generally involve administering to an individual infected with the lentivirus an effective amount of a compound that induces Vpr loss and/or that inhibits PPIase activity of a protein that catalyzes cis-trans isomerization of cis-peptidylprolyl bonds in Vpr.

Methods of identifying agents for inhibiting lentivirus replication

An endotracheal tube and a method for using an endotracheal tube are disclosed. In one embodiment, the endotracheal tube includes a tubular member including a distal end and a proximal end. The endotracheal tube also includes a plurality of visually distinct regions that is closer to the proximal end than the distal end. Each visually distinct region is spaced from other visually distinct regions, and each of the distinct region comprises a respectively different color.

Endotracheal tube

Provided is a composition comprising a Vpr polypeptide conjugated to a therapeutic molecule. Preferably, the Vpr comprises synthetic Vpr. The therapeutic molecule can comprise any molecule capable of being conjugated to Vpr or a fragment thereof, including a polypeptide, a polynucleotide, and/or a toxin. The invention additionally provides a method for delivering a molecule into a cell. The method comprises contacting the cell with a conjugate comprising a Vpr polypeptide conjugated to the molecule. The invention further provides a method for modulating the expression of a transgene in a cell, a method for killing a target cell population in a subject, a method for increasing the sensitivity of cells to radiation therapy, and a method for inhibiting cell proliferation.

Compositions and methods for delivery of a molecule into a cell

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Davis, CA, United States of America

Michael P Sherman