Wisconsin Alumni Research Foundation

Michael P Schwartz

William Leo Murphy

James A Thomson

Zhonggang Hou

Igor I Slukvin

Jue Zhang

Nicholas E Propson

Gene Ichiro Uenishi

Stefan Zorn

Eric Huy Dang Nguyen

Ngoc Nhi Le

David Belair

Matthew Brian Parlato

William T Daly

Gene I Uenishi

Matthew Zanotelli

Hamisha Ardalani

Ngoc Nhi Thi Le

Charles D Page

David Gregory Belair

The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.

Derivation of human microglia from pluripotent stem cells

The present invention relates to three-dimensional (3D) tissue constructs and methods of using such 3D tissue constructs to screen for neurotoxic agents. In particular, provided herein are methods of producing and using complex, highly uniform human tissue models comprising physiologically relevant human cells, where the tissue models have the degree of sample uniformity and reproducibility required for use in quantitative high-throughput screening applications.

Human pluripotent stem cell-based models for predictive developmental neural toxicity

Patterned hydrogel arrays and methods of preparing patterned hydrogel arrays are disclosed. Advantageously, the methods used to prepare the patterned hydrogel arrays allow for controlling individual hydrogel spot conditions such as hydrogel spot modulus, hydrogel spot ligand identity and hydrogel spot ligand density, which allows for preparing a wide range of hydrogel spots in a single array format. Patterned hydrogel arrays can also be formed to include hydrogel-free pools surrounded by hydrogel. Additionally, the patterned hydrogel arrays of the present disclosure support the culture of a range of cell types. The patterned hydrogel arrays offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation.

Method for forming hydrogel arrays using surfaces with differential wettability

Hydrogel Compositions and methods of using hydrogel compositions are disclosed. Advantageously, the hydrogel compositions offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation. In particularly suitable embodiments, the hydrogel compositions of the present disclosure may be used to promote tubulogenesis of endothelial cells.

Hydrogel compositions for use in promoting tubulogenesis

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Madison, WI, United States of America

Michael P Schwartz