E.i. Dupont De Nemours and Company

Michael P Perry

Edwin R Hendrickson

Abigail K Luckring

Sharon Jo Keeler

Eric R Choban

Robert Donald Fallon

Christina S Stauffer

Scott Christopher Jackson

T Joseph Dennes

James M Prober

Kostantinos Kourtakis

Qiong Cheng

Larry Eugene Steenhoek

Richard C Ebersole

Luan Tao

Albert W Alsop

Paul Douglas Stull

Rudy J Dam

Raymond E Jackson

Kevin Michael Croker

Linda L Hnatow

Bogdan Szostek

Michael B Damore

Neel V Neelkantan

Sheryl Wolstenholme

Scott Christopoher Jackson

Sheryl M Anzek

Luis Baez

Xueping Jiang

Xiumin Cui

David Alan Suchanec

Rachel L Hardie

Berardino D'Achille

Sheryl M Anyzek

Berardino D'achille

Methods and compositions are provided wherein microorganisms are used to alter the interface of hydrocarbons and hydrocarbon-coated surfaces to increase oil recovery, for improved bioremediation and/or to benefit pipeline maintenance.

Altering the interface of hydrocarbon-coated surfaces

Methods and compositions are provided wherein microorganisms are used to alter the interface of hydrocarbons and hydrocarbon-coated surfaces to increase oil recovery, for improved bioremediation and/or pipeline maintenance.

Disclosed herein are processes for collecting nucleic acids from particulate samples. One embodiment disclosed herein relates to the use of ultrasonic energy to simultaneously shear large nucleic acid molecules and large particulates to very small sizes prior to or during a chemical binding step to a nucleic acid binding surface. Another embodiment involves crushing the nucleic acid binding surface prior to eluting the bound nucleic acid molecules to enable better wetting of the nucleic acid binding surface and easier diffusion of bound nucleic acid molecules out of the nucleic acid binding surface.

Methods for improved DNA release from binding substrates and/or decreasing PCR inhibition in pathogen detection

Chemical compounds that are N-acyl amino acids or derivatives thereof having long chain N-acyl groups were found to have oil-releasing activity. Solutions containing these compounds may be introduced into oil reservoirs or onto oil-contaminated surface sites to release oil from oil-coated surfaces. The released oil may be recovered for further processing or waste disposal.

Oil release with amino acid-based compounds having a long chain N-acyl group

Chemical compounds that are N-lauroyl amino acids containing a polyethylene oxide segment were found to have oil-releasing activity. Solutions containing these compounds may be introduced into oil reservoirs or onto oil-contaminated surface sites to release oil from oil-coated surfaces. The released oil may be recovered for further processing or waste disposal.

Oil release with polyethylene oxide segment-containing N-lauroyl amino acid-based compounds

Enriched steady state microbial consortiums for microbial enhanced oil recovery and in situ bioremediation of hydrocarbon-contaminated sites, under anaerobic denitrifying conditions, are disclosed.

Steady state anaerobic denitrifying consortium for application in in-situ bioremediation of hydrocarbon-contaminated sites and enhanced oil recovery

Disclosed herein is introduction of different nutrient solution formulations to a subterranean target site to prevent biofilm formation and biomass aggregation at the sand face of an injection well bore during MEOR or bioremediation processes. A first nutrient solution formulation supports growth of introduced microorganisms and a second nutrient solution formulation is used to promote biofilm formation to allow plugging of pores and channels in the subterranean target site, for MEOR or bioremediation.

Prevention of biomass aggregation at injection wells

Chemical compounds that are N-lauroyl amino acids or derivatives thereof were found to have oil-releasing activity. Solutions containing these compounds may be introduced into oil reservoirs or onto oil-contaminated surface sites to release oil from oil-coated surfaces. The released oil may be recovered for further processing or waste disposal.

Oil release with N-lauroyl amino acid-based compounds

The present disclosure relates to developing an enriched steady state microbial consortium capable of modifying crude oil components of an oil reservoir under anaerobic denitrifying conditions. The steady state consortium may be used to improve oil recovery.

Method of improving oil recovery from an oil reservoir using an enriched anaerobic steady state microbial consortium

The present disclosure relates to isolation and identification of uniquestrains that can grow on crude oil under denitrifying conditions and are useful in oil recovery.

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The present disclosure relates to isolation, identification and application ofstrain AL9:8 which grows on crude oil as the sole carbon source under denitrifying anaerobic conditionsstrain AL9:8 can be used alone or in concert with other microorganisms to improve oil recovery, bioremediation of oil or hydrocarbons in contaminated soil, ground water or bodies of water, such as lakes, rivers or oceans and/or applying to method for promoting oil pipeline maintenance, removing a build up hydrocarbon on the intersurfaces of the pipeline.

A microsphere for use in a bioassay comprising a glass core coated with a nanoparticle composite comprising a bioactive probe is provided. The nanoparticle composite coating enhances the density of bioprobe loading on the surface of the microspheres, resulting in enhanced dynamic range and sensitivity in bioassays. The particle may be used in detection systems where resonant light scattering properties of the particle are useful.

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Landenberg, PA, United States of America

Michael P Perry